Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,092 results for: pcdna3.1

Clear keywords
Showing: 2241 - 2260 of 7092 results

  4. pcDNA3.1-KIT-D816V-V5/HIS

    Plasmid
    #236010
    Purpose
    expression of the D816V kinase defective mutant variant of human KIT that is associated with Acute Myeloid Leukemia and MASTSYS
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human KIT-D816V receptor tyrosine kinase, full length (KIT Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D816V substitution
    Promoter
    CMV
    Available since
    May 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    236010-plasmid-map-sequence-id-511408

    Enlarge

  5. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Publication
    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

    Enlarge

  6. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid
    #236008
    Purpose
    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835N substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236008-plasmid-map-sequence-id-469587

    Enlarge

  10. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid
    #207153
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAQ (GNAQ Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position F(124)E
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207153-plasmid-map-sequence-id-410285

    Enlarge

  11. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNAS (GNAS Human)
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207158-plasmid-map-sequence-id-410280

    Enlarge

  12. pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

    Plasmid
    #207159
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNG1
    Expression
    Mammalian
    Mutation
    N-terminally tagged with LSS-mKATE2
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207159-plasmid-map-sequence-id-410272

    Enlarge

  13. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

    Plasmid
    #207160
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA
    Depositor
    Dominique Schols, Tom Van Loy
    Publication
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GNG2 (GNG2 Human)
    Expression
    Mammalian
    Mutation
    N-terminally tagged with LSS-mKATE2
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207160-plasmid-map-sequence-id-410278

    Enlarge

  14. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid
    #205568
    Purpose
    RasAR negative-control mutant plasmid. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR(R89L)-NES (RAF1 Synthetic, Human)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Arg 89 mutated to Leu in Raf1 RBD region.
    Promoter
    CMV
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205568-plasmid-map-sequence-id-409421

    Enlarge

  15. EGFP Rab11a WT pcDNA3.1 Zeo (+)

    Plasmid
    #206079
    Purpose
    Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes
    Depositor
    Annette Dolphin
    Publication
    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB11A (RAB11A Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    206079-plasmid-map-sequence-id-410291

    Enlarge

  16. pcDNA3.1(-) HuTF-Fc-His tag

    Plasmid
    #183253
    Purpose
    Soluble expression of the extracellular domains of human tissue factor (TF)-Fc
    Depositor
    Alexander McLellan
    Insert
    Tissue factor (F3 Human)
    Tags
    8xHis
    Expression
    Mammalian
    Available since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183253-plasmid-map-sequence-id-369717

    Enlarge

Showing: 2241 - 2260 of 7092 results