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pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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mIFP-Vimentin-7

Plasmid

#56242

Purpose

Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Vimentin (VIM Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-mEGFP

Plasmid

#115911

Purpose

donor to insert EGFP at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-mEGFP (TOMM20 Human)

Use

Donor template

Tags

EGFP

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-CENPB-N-22

Plasmid

#56215

Purpose

Localization: Nucleus/Centromeres, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CENPB (CENPB Human)

Tags

mIFP

Expression

Mammalian

Mutation

aa 1-169 of CENPB (DNA Binding Domain)

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Clathrin-15

Plasmid

#56249

Purpose

Localization: Clathrin Vesicles, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001834.3 (CLTB Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-Actin-C-18

Plasmid

#56244

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Actin (ACTB Human)

Tags

mIFP12

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Actin-C-18

Plasmid

#56211

Purpose

Localization: Actin, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

NM_001101.3 (ACTB Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP12-LASP1-C-10

Plasmid

#56257

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LASP1 (LASP1 Human)

Tags

mIFP12

Expression

Mammalian

Mutation

A209V

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mIFP-Zyxin-6

Plasmid

#56243

Purpose

Localization: Focal Adhesions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Zyxin (ZYX Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-H1-10

Plasmid

#56222

Purpose

Localization: Nucleus/Histones, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

H1 (H1f0 Mouse)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mIFP-Keratin-17

Plasmid

#56225

Purpose

Note- This plasmid contains Keratin 18. Localization: Intermediate Filaments, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Keratin (KRT18 Human)

Tags

mIFP

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBS-f1(+)-SEC61B-dTomato

Plasmid

#115913

Purpose

donor to insert dTomato at the SEC61B locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SEC61B-dTomato (SEC61B Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-TOMM20-dTomato

Plasmid

#115914

Purpose

donor to insert dTomato at the TOMM20 locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

TOMM20-dTomato (TOMM20 Human)

Use

Donor template

Tags

dTomato

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-Cx26-7

Plasmid

#56216

Purpose

Localization: Gap Junctions, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Cx26 (Gjb2 Rat)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2 puro

Plasmid

#98290

Purpose

Variant of lentiCRISPRv2 that confers puromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

EF-1a

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRM049 His-TEV-Cas9 triNLS 2C

Plasmid

#196245

Purpose

Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

CRISPR

Tags

HA, His6 (N terminal on backbone), NLS, Nucleopla…

Expression

Bacterial

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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