We narrowed to 8,785 results for: sgrna

1. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA)

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN227

   Plasmid

   #127222

   Purpose

   BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Cas9, WUS2, AtSTM, sgRNA
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD428

   Plasmid

   #202081

   Purpose

   Cas9/sgRNA plasmid targeting the C-terminus of Myh9

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIF1079

   Plasmid

   #237445

   Purpose

   Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

   Depositor

   IIya Finkelstein

   Article

   Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

   Inserts

   SpCas9
   Efe1-RT
   EMX1 sgRNA
   Efe1-ncRNA targeting EMX1
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, H1, and U6

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIW601-KmCRISPR

   Plasmid

   #98907

   Purpose

   K. marxianus CRISPR Plasmid for sgRNA cloning

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC1640

   Plasmid

   #158719

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM114

   Plasmid

   #127211

   Purpose

   BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   WUS2, IPT, sgRNA, Luc
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZR060_CROPseq-sgCtrl-CRISPRi

    Plasmid

    #180269

    Purpose

    Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-TJP1

    Plasmid

    #227299

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TJP1 (TJP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMM113

    Plasmid

    #127210

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, AtSTM, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJMP8823

    Plasmid

    #220901

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJMP8930

    Plasmid

    #220905

    Purpose

    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEditC

    Plasmid

    #207532

    Purpose

    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEditA-RY

    Plasmid

    #207533

    Purpose

    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEditC-RY

    Plasmid

    #207534

    Purpose

    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    

    Use

    CRISPR; Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEVA-gRic6T

    Plasmid

    #106401

    Purpose

    E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

    Depositor

    Sheng Yang

    Article

    Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pj23119

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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