We narrowed to 2,327 results for: PUC19

1. pIG-836_NY-ESO-1_TCR_FAS/OX40

   Plasmid

   #207499

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/OX40, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIG-833_NY-ESO-1_TCR_FAS/CD28

   Plasmid

   #207496

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   FAS/CD28, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIG-671_HA-GD2-28z_CAR_tNGFR

   Plasmid

   #207484

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, HA-GD2-28z_CAR (NGFR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-874_HA-GD2-28z_CAR_IL2RA

   Plasmid

   #207504

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   IL2RA, HA-GD2-28z_CAR (IL2RA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

   Plasmid

   #202762

   Purpose

   Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

   Plasmid

   #239102

   Purpose

   Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

   Depositor

   Karl Wahlin

   Article

   Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

   Insert

   POU4F2 (POU4F2 Human)
   

   Use

   Donor plasmid for integration into the human geno…

   Tags

   p2A-tdTomato-p2A-Thy1.2

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous POU4F2

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. P527_POU4F2-p2A-mNeonGreen-h2b

   Plasmid

   #239103

   Purpose

   Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   POU4F2 (POU4F2 Human)
   

   Use

   Tags

   p2A-mNeonGreen-h2b

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous POU4F2

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYX-N-Puro-mNeon-TSC22D1

   Plasmid

   #222667

   Purpose

   endogenously tag TSC22D1 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D1 (TSC22D1 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYX-N-Blast-miniTurbo-TSC22D2

   Plasmid

   #222669

   Purpose

   endogenously tag TSC22D2 with N-terminal miniTurbo

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Use

   Tags

   miniTurbo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIG-748_HA-GD2-28z_CAR_BATF

    Plasmid

    #207488

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIG-805_HA-GD2-28z_CAR_BATF-RFP

    Plasmid

    #207492

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF-RFP, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIG-755_HA-GD2-28z_CAR_RFP-JUN

    Plasmid

    #207494

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-JUN, HA-GD2-28z_CAR (JUN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIG-837_NY-ESO-1_TCR_tFAS

    Plasmid

    #207500

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tFAS, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTR-378 tNGFR-P2A-CCR7

    Plasmid

    #186072

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CCR7 (CCR7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTR-450 tNGFR-P2A-CXCR4

    Plasmid

    #186078

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTPT

    Plasmid

    #1962

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 1998 Sep 15;12(18):2842-51.

    Insert

    TPT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    2 copies of 20bp artificial nucleosome positionin…

    Promoter

    Available since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pWAP-HGF

    Plasmid

    #83503

    Purpose

    Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

    Depositor

    Marta Gallego, Lothar Hennighausen

    Article

    Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

    Insert

    HGF (Hgf Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    also contains beta-globin sequences from pUC198

    Promoter

    mouse WAP (whey acidic protein)

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

    Plasmid

    #112022

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha/beta into TRBC1 HDRDT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NYESO alpha into TRAC HDRT Source (pTR 223)

    Plasmid

    #112023

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge