We narrowed to 41,868 results for: tro

1. pCW57.1 tTA UQCRC2

   Plasmid

   #177978

   Purpose

   All-in-one dox-repressible expression of cDNA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   UQCRC2 (UQCRC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Silent point mutations to UQCRC2 sgRNA target

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-SspB-HaloTag-TIAM1-DHPH

   Plasmid

   #176127

   Purpose

   Heterodimerization with iLID, Rac1 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   TIAM1 (TIAM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

   Plasmid

   #74309

   Purpose

   Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Human, Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM643

   Plasmid

   #112533

   Purpose

   pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

   Depositor

   Timothy Lu

   Article

   Mimee et al Science. 2018 May 25;360(6391):915-918.

   Inserts

   HrtR
   ChuA
   luxCDABE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17P

   Promoter

   J23107, PL(HrtO), and ProD

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV[Exp]-Neo-EF1A>Tet3G

   Plasmid

   #184379

   Purpose

   The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Tet3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-lyzC-mcherry-2a-CDK2

   Plasmid

   #121131

   Purpose

   neutrophil specific expression

   Depositor

   Qing Deng

   Article

   Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

   Insert

   dre-WT CDK2 (cdk2 Zebrafish)
   

   Use

   Tags

   mcherry-2a

   Expression

   Bacterial

   Mutation

   Promoter

   neutrophil specific lyzC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. xlox dNGFR-TERT

   Plasmid

   #69805

   Purpose

   MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

   Depositor

   David Ott

   Article

   Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

   Insert

   hTERT reverse transcriptase (TERT Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mutation

   Promoter

   MLV promoter

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-neo-TET

   Plasmid

   #51286

   Purpose

   expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   U6 (RNU6-1 Human)
   

   Use

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Mutation

   Promoter

   U6 upstream enhancer sequence

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SspB-HaloTag-DHPH-TIAM1

    Plasmid

    #176114

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SspB-mCherry-DHPH-TIAM1

    Plasmid

    #176104

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBactin-Halo-Tag

    Plasmid

    #196898

    Purpose

    Expression of Halo-Tag. Use as control for Halo-tag fusions

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Halo-Tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SspB-iRFP-DHPH-TIAM1

    Plasmid

    #176118

    Purpose

    Heterodimerization with iLID, Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-SSeCKs T751A

    Plasmid

    #107142

    Purpose

    full-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vector

    Depositor

    Heidi Hehnly

    Article

    Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

    Insert

    SSeCKs T751A (Akap12 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed threonine 751 to alanine

    Promoter

    gag

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-ΔN-RNMT

    Plasmid

    #112708

    Purpose

    Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNMT (RNMT Human)
    

    Use

    Tags

    FLAG and HIV Rev nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    deleted amino acids 2-120, mutated active-site D2…

    Promoter

    CMV

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. SHIP2 WT pBABE-His-Xpress

    Plasmid

    #126584

    Purpose

    Retroviral infection of MEF cells (puromycin selection)

    Depositor

    Christophe Erneux

    Article

    Zhang et al Cell Signal. 2007 Oct;19(10):2194-200. d

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Retroviral

    Tags

    His Xpress (N-terminal)

    Expression

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCIBN-TetR

    Plasmid

    #103813

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-TetR (CIB1, tetR Mustard Weed, E. coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs-hs-3xHA-delta6xNHL-LIN-41

    Plasmid

    #52722

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 593–868

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-gfap-sgRNA

    Plasmid

    #65566

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap sgRNA (gfap Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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