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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,612 results for: ubiquitin

Showing: 2301 - 2320 of 2612 results

  1. pSpG-PPE

    Plasmid
    #170130
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9-SpG(H840A)-M-MLV
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…
    Promoter
    maize Ubiquitin-1
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170130-plasmid-map-sequence-id-333605

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  2. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid
    #131232
    Purpose
    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    DNA polymerase iota (POLI Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains inactivating mutations in both ubiquitin…
    Promoter
    CMV enhancer + CMV promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131232-plasmid-map-sequence-id-267278

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  3. pOD2045-intControl

    Plasmid
    #100005
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2044-intDEG. Expression is controlled by Pelt-2 (intestine specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100005-plasmid-map-sequence-id-193781

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  6. MtPT4-p3

    Plasmid
    #160003
    Purpose
    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.
    Depositor
    Sam Brockington
    Article
    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d
    Inserts
    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160003-plasmid-map-sequence-id-323050

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  10. pH-ABE8e-spG

    Plasmid
    #200775
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9-spG(D10A)-TadA8e
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200775-plasmid-map-sequence-id-399599

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  11. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    89369-plasmid-map-sequence-id-177235

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  15. pOD2049-csnControl

    Plasmid
    #100007
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100007-plasmid-map-sequence-id-193784

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  19. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225955-plasmid-map-sequence-id-446861

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  20. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva, Evan Reid
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225938-plasmid-map-sequence-id-446791

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Showing: 2301 - 2320 of 2612 results