We narrowed to 17,362 results for: ef1;

1. pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

   Plasmid

   #39455

   Depositor

   Hongjun Song

   Article

   Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

   Insert

   HA-hTet1CDmu (TET1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1418–2136 with mutations H1672 to Y, 1674D to A

   Promoter

   EF1a

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

2. 61426-hU6-shmFmr1-EF1a-mCherry

   Plasmid

   #157859

   Purpose

   Expresses a shRNA targeting mouse Fmr1 gene

   Depositor

   Xinyu Zhao

   Article

   Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-EF1-IRES-NEO mutant ITR

   Plasmid

   #67905

   Purpose

   PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-FDIO-ArchT-GFP

   Plasmid

   #124640

   Purpose

   AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   ArchT
   

   Use

   AAV; Flp/frt

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-Ef1a-RPS27A-UTR-PP7-WPRE

   Plasmid

   #198339

   Purpose

   PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   RPS27A-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-mTagBFP-T2A-TetOn3G

   Plasmid

   #179888

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   mTagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

   Plasmid

   #203845

   Purpose

   Flp-dependent enzymatically inactive Cre and mRuby3 expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dCre
   

   Use

   AAV

   Tags

   mRuby3-2A

   Expression

   Mutation

   Enzymatically inactive Cre

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-FLEX-ArchT-GFP

   Plasmid

   #58851

   Purpose

   AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Insert

   ArchT-GFP
   

   Use

   AAV and Cre/Lox

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

    Plasmid

    #122102

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1α-IRES-EGFP-hMCIDAS

    Plasmid

    #149696

    Purpose

    Expresses untagged human MCIDAS in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    MCIDAS (MCIDAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lv EF1a HP1cs-Frb1x PGK Puro

    Plasmid

    #102808

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb1x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQLL-Tc1-8B_DR[EF1α-copGFP]

    Plasmid

    #218327

    Purpose

    Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA

    Plasmid

    #37087

    Purpose

    Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibition

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    NpHR3.0-EYFP
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128587

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. P-Ef1a-mCherry-T2A-RPL22-1xV5

    Plasmid

    #170324

    Purpose

    Expresses V5 and mCherry tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-EF1a-MCS-hPOT1_IRES-blast

    Plasmid

    #166411

    Purpose

    express hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Use

    Lentiviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    ef-1a

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

    Plasmid

    #170228

    Purpose

    Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    Map2k7-MAPK9 fusion (MAPK9, Map2k7 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Fusion

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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