We narrowed to 17,478 results for: igh
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Plasmid#52068PurposeExpresses full-length Nidogen-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNID1 (NID1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag
Plasmid#209663PurposeExpression of iATPSnFR2, high affinityDepositorInsertiATPSnFR2.S29W.HaloTag
UseAAVMutationS29W.A95KPromoterhSynAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio-His
Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
TagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2-N
Plasmid#41645PurposeExpresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)DepositorInsertmNeptune2-Dronpa145N
ExpressionMammalianMutationK145N on Dronpa (allows control of photochromic F…PromoterCMVAvailable SinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
ePB-PURO-TT-PA-CRE
Plasmid#179584PurposeePiggyBac vector to conditionally express using DOX a light-inducible Cre-recombinase enzyme that takes advantage of the Magnets dimerization system (Magnet-CRE)DepositorInsertPA-MAG-CRE
ExpressionMammalianPromoterTREAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-h_bPAC_cmyc
Plasmid#28134Purposeoptogenetic tool for light modulation of cellular cAMPDepositorInserthumanized photoactivated adenylyl cyclase
UseIn vitro transcriptionTagsMycAvailable SinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-ZO1-C-14
Plasmid#55916PurposeLocalization: Tight Junctions, Excitation: 559, Emission: 600DepositorAvailable SinceMarch 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralExpressionMammalianAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN41
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag
Plasmid#209659PurposeExpression of iATPSnFR2, high affinityDepositorInsertiATPSnFR2.S29W.HaloTag
UseAAVMutationS29W.A95KPromoterGFAPAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SlugCas9-HF
Plasmid#163796PurposeExpresses highly specific SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianMutationSlugCas9 (R247A, N415A, T421A, R656A)Available SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChRger2-TS-EYFP
Plasmid#127237PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterhSyn1Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pyc126m
Plasmid#158777Purposeribosome-tagged GCaMP for soma localizationDepositorInsertGCaMP6m-RPL10a
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
hDHFR
Plasmid#184707Purposebacterial expression of human DHFRDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pyc111s
Plasmid#158781Purposeribosome-tagged, Cre-dependent GCaMP for soma localizationDepositorInsertGCaMP6s-RPL10a
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only