We narrowed to 9,269 results for: ef1

Showing: 4681 - 4720 of 9269 results

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pLV V5-nurim

Plasmid

#175110

Purpose

Lentiviral expression of V5-tagged mouse Nrm

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Nrm (Nrm Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Tmem53-V5

Plasmid

#175142

Purpose

Lentiviral expression of mouse Tmem53-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem53 (Tmem53 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pONSY-Lifeact:mCherry

Plasmid

#111876

Purpose

This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Lifeact fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_F(ab')2

Plasmid

#105860

Purpose

Expression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 F(ab')2 (Ighg3 Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_WT_ZFP64

Plasmid

#118661

Purpose

Express human wild type ZFP64 gene

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Insert

ZFP64 (ZFP64 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB221

Plasmid

#68612

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_NEDD9-1/1

Plasmid

#91498

Purpose

Protein expression and purification of human SH3 domain construct NEDD9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NEDD9-1/1 (NEDD9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGWKS6

Plasmid

#139413

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mMaroon1

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2

Plasmid

#110735

Purpose

Recombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

H2B-mOrange2

Use

Other

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Expression

Mammalian

Promoter

hEF1alpha

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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p413TEF-CARKL

Plasmid

#51747

Purpose

overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236364

Purpose

Overexpression of dLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pJL179

Plasmid

#243688

Purpose

Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1 (SUPV3L1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FOidr_097

Plasmid

#244649

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_097, derived from human fusion protein LEF1_TAF8

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_097

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FOidr_096

Plasmid

#244648

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_096, derived from human fusion protein LEF1_TAF8

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_096

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_FOidr_095

Plasmid

#244647

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_095, derived from human fusion protein LEF1_TAF8

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_095

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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iSM_PPL2-IP

Plasmid

#235989

Purpose

Expresses iSM_PPL2 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM_PPL2

Use

CRISPR

Expression

Mammalian

Mutation

replacement of 5 amino acids of Smac-Cas9 PL2 wit…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236375

Purpose

Overexpression of dFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dFnCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236363

Purpose

Overexpression of endAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

endAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, S542R, K548R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236362

Purpose

Overexpression of HyperdAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, D256R, E321R, E371R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236361

Purpose

Overexpression of dAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dAsCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235309

Purpose

ComMAND open-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-Dok1-Clover

Plasmid

#215456

Purpose

Expression vector with Dok1 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-Dok1

Plasmid

#215458

Purpose

Expression vector with Dok1

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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EFS-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235310

Purpose

ComMAND closed-loop circuit regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pC2.4

Plasmid

#226557

Purpose

NATURA gene encoding plasmid

Depositor

Dave Wee

Article

Tabaglio et al Mol Ther Nucleic Acids. 2025 Jan 7;36(1)

Insert

NATURA reporter gene

Use

Luciferase; Piggybac

Tags

EGFP and Flag

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 APE1 SO

Plasmid

#227192

Purpose

Inducible lentiviral expression of SO (Slow Off) APE1

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)

Expression

Bacterial and Mammalian

Mutation

D283A D308A

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS-HsProm1s1(W795R)-StayGold

Plasmid

#211334

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Mutation

Changed Trp-795 to Arg

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pToxAmp1

Plasmid

#218285

Purpose

Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism

Depositor

Claudia Vickers

Article

Evans et al bioRxiv 2024.01.30.578080

Insert

HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)

Expression

Yeast

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#216098

Purpose

CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Puro_CI-hCOL4A5-Nluc_R1683X

Plasmid

#198111

Purpose

lentiviral expression of hCOL4A4_R1683Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1683X

Promoter

EF1a

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_C1684X

Plasmid

#198112

Purpose

lentiviral expression of hCOL4A4_C1684X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C1684X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_C29X

Plasmid

#198107

Purpose

lentiviral expression of hCOL4A5_C29X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

C29X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_R1563X

Plasmid

#198109

Purpose

lentiviral expression of hCOL4A4_R1563Xin mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

R1563X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc_S36X

Plasmid

#198108

Purpose

lentiviral expression of hCOL4A4_S36X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Mutation

S36X

Promoter

EF1a

Available Since

March 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Puro_CI-hCOL4A5-Nluc

Plasmid

#198106

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Use

Lentiviral

Tags

Nluc

Promoter

EF1a

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD424-Rabex-5

Plasmid

#197043

Purpose

Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

Insert

Rabex-5 (RABGEF1 Bovine)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PlmdCas12e VPR

Plasmid

#188516

Purpose

Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

NLS-FLAG-VPR

Expression

Mammalian

Mutation

D659A/E756A/D922A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD2604

Plasmid

#175088

Purpose

Mammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).

Depositor

Dirk Dittmer

Article

Alzhanova et al J Virol. 2021 Nov 9;95(23):e0145921. doi

Insert

KSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)

Expression

Mammalian

Mutation

S644A

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4681 - 4720 of 9269 results

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