We narrowed to 87,711 results for: lias

1. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OSCA1.8

   Plasmid

   #136594

   Purpose

   Expresses the mechanosensitive channel OSCA1.8 in mammalian cells

   Depositor

   Ardem Patapoutian

   Article

   Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

   Insert

   OSCA1.8 (AT1G32090 Mustard Weed)
   

   Use

   Tags

   pIRES2-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FKBP_LD 0_TEV

   Plasmid

   #58877

   Purpose

   MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX307 LacZ

   Plasmid

   #117730

   Purpose

   Open reading frame vector encoding LacZ

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-PDZD8HA_sh_resistant

   Plasmid

   #105006

   Purpose

   Mammalian Expression of PDZD8, sh resistant

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   PDZD8 (Pdzd8 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The sequence recognized by shPDZD8 (TRCN000043105…

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCyterm-mScarlet-I3_N1

   Plasmid

   #189777

   Purpose

   Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   Cyterm-mScarlet-I3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV_PACLight1

    Plasmid

    #197864

    Purpose

    Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

    Depositor

    Tommaso Patriarchi

    Article

    Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

    Insert

    PACLight1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

    Plasmid

    #69906

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OSCA1.1

    Plasmid

    #136592

    Purpose

    Expresses the mechanosensitive channel OSCA1.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.1 (AT4G04340 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXL004-ishRNA-scramble

    Plasmid

    #36311

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of scramble
    

    Use

    Lentiviral and RNAi

    Tags

    EF1a-TetR-IRES-RFP-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    TetO-H1

    Available since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cry2(1-531)-mCh-BAXS184E

    Plasmid

    #117238

    Purpose

    Light activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    Cry2531-mCh-BAXS184E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HsNXF-1_K

    Plasmid

    #146740

    Purpose

    Mammalian Expression of HsNxf-1

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsNxf-1 (NXF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S144G relative to NM_006362.4

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OSCA3.1

    Plasmid

    #136596

    Purpose

    Expresses the mechanosensitive channel OSCA3.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA3.1 (ERD4 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBI-eGFP-Rab31

    Plasmid

    #200265

    Purpose

    Expression of eGFP-Rab31 (human) in mammalian cells

    Depositor

    Guangpu Li

    Article

    Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

    Insert

    Rab31 (human cDNA) (RAB31 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGH183_MS2-AIDΔDead-Hygro

    Plasmid

    #85414

    Purpose

    lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔDead and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.T7 delta-NFAT3

    Plasmid

    #28224

    Depositor

    Sebastien Jauliac

    Article

    Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

    Insert

    Delta NFAT3 (NFATC4 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    hNFAT3 WT deleted amino acids 1-342

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLX307 hcRed1

    Plasmid

    #117732

    Purpose

    Open reading frame vector encoding hcRed1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    hcRed1
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAL101

    Plasmid

    #22474

    Purpose

    Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBI-eGFP-Rab22

    Plasmid

    #200264

    Purpose

    Expression of eGFP-Rab22 (human) in mammalian cells

    Depositor

    Guangpu Li

    Article

    Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

    Insert

    Rab22a (human cDNA) (RAB22A Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX311 hcRed1

    Plasmid

    #117733

    Purpose

    Open reading frame vector encoding hcRed1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    hcRed1
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OSCA4.1

    Plasmid

    #136597

    Purpose

    Expresses the mechanosensitive channel OSCA4.1 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA4.1 (AT4G35870 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pICSL90002

    Plasmid

    #68261

    Purpose

    Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter: U6-26 (Arabidopsis thaliana)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MBP-CRY1-PHR

    Plasmid

    #26035

    Depositor

    Johann Deisenhofer

    Article

    Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    CRY1 (CRY1 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pRK-flag-USP19 C506S

    Plasmid

    #78584

    Purpose

    Expression flag-USP19 C506S in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 C506S (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 506 cysteine residue to serine

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pMS2CP(V29I)-myc-T2A-tagRFP

    Plasmid

    #140260

    Purpose

    Expresses MS2CP(V29I) in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Coat protein of bacteriphage MS2
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNTI826

    Plasmid

    #216634

    Purpose

    cpTurboID (V5) for AAV production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge