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pET30b-7D-SrtA

Plasmid

#105600

Purpose

expresses 7D sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7D mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-7xHalo7::CAAX

Plasmid

#87647

Purpose

For creating drosophila transgenics expressing UAS-7xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

7xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCabp5-DsRed

Plasmid

#11157

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

Cabp5 promoter (Cabp5 Mouse)

Tags

DsRed2

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG2

Plasmid

#129617

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-ER(T2)CreER(T2)

Plasmid

#32649

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
ER(T2)CreER(T2)

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJL005

Plasmid

#92327

Purpose

Express FtsZ tagged with mEos3.2 in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-PLEKHG1

Plasmid

#129616

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

PLEKHG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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UAS-myr::4xCLIPf

Plasmid

#87635

Purpose

For creating drosophila transgenics expressing UAS-myr4x::CLIPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xCLIPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDB2

Plasmid

#18954

Depositor

Michele Calos

Article

Keravala et al J Gene Med. 2006 Aug . 8(8):1008-17.

Insert

phiC31 attB

Expression

Mammalian

Available Since

Oct. 14, 2008

Availability

Academic Institutions and Nonprofits only

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FUap97GW (C4)

Plasmid

#74020

Purpose

lentiviral expression of SAP97alpha-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

SAP97 alpha

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

hUbC

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-NeoR

Plasmid

#207175

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-FLPo-DsRedX

Plasmid

#32650

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

FLPo
DsRedX

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
LexAop2-myr::4xSNAPf

Plasmid

#87638

Purpose

For creating drosophila transgenics expressing LexAop2-myr4x::SNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pJV0010

Plasmid

#205132

Purpose

Expression of 1xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

1xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaIg

Plasmid

#22629

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta Ig (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of Ig domain

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin

Plasmid

#84673

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Tags

GST

Expression

Bacterial

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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MyrPalm-BKAR

Plasmid

#14878

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) TRIM12B

Plasmid

#87079

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12B (Trim5 Mouse)

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30A

Plasmid

#87081

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30A (Trim30a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CGG-R12-KIRV

Plasmid

#63668

Purpose

CGG-R12-KIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CGG-R12-KIRV RNAP

Mutation

Q744K, L747V, N748H, L749I, R756E, L757M, H772R, …

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Citrine-pBAD

Plasmid

#54772

Purpose

Localization: Protein Expression Vector , Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

6xHis and Citrine

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-Asef2

Plasmid

#129610

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

Asef2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-mCherry-Myc

Plasmid

#32637

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

mCherry

Use

Rabies virus

Tags

6xMyc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pBScMXT + dSlo

Plasmid

#16173

Depositor

Larry Salkoff

Article

Wei et al Neuron. 1994 Sep . 13(3):671-81.

Insert

Drosophila melanogaster calcium activated potassium channel (slo Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
p6239 pGL4-HPV16-LCR (nt 7000-85)-luciferase

Plasmid

#32889

Depositor

Peter Howley

Article

Ottinger et al Virology. 2009 Dec 20;395(2):161-71.

Insert

LCR HPV16

Promoter

LCR

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-FGD5

Plasmid

#129613

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

FGD5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-WT-CTGA-R13-AKSIRV

Plasmid

#63627

Purpose

CTGA-R13-AKSIRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSIRV RNAP

Tags

n/a

Mutation

V725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …

Promoter

T5/Lac

Available Since

June 3, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSADdeltaG-GCaMP3-DsRedX

Plasmid

#32645

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GCaMP3
DsRedX

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pRS306:VN

Plasmid

#37555

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Venus N-term (aa 1-272)

Expression

Bacterial

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAG505-H2B-frFAST

Plasmid

#160056

Purpose

Expresses H2B-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

H2B-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Septin 11 isoform IV

Plasmid

#120320

Purpose

Septin 11 isoform IV mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform I

Plasmid

#120314

Purpose

Septin 11 isoform I mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
OMP-SpH-ACNF TV

Plasmid

#15513

Depositor

Peter Mombaerts

Article

Bozza et al Neuron. 2004 Apr 8. 42(1):9-21.

Insert

olfactory marker protein (Omp Mouse)

Use

Mouse Targeting

Mutation

OMP coding region and 3' end were replaced b…

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pQE-WT-CTGA-R13-AKSRV

Plasmid

#63622

Purpose

CTGA-R13-AKSRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

CTGA-R13-AKSRV RNAP

Expression

Bacterial

Mutation

V725A, Q744K, L747I, N748S, R756T, Q758K, H772R, …

Promoter

T5/Lac

Available Since

July 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRiboS BsaHind

Plasmid

#36253

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pTricHis-ILY-WT-Y336W

Plasmid

#160515

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y336W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ZE10 sonic hedgehog pT7TS

Plasmid

#17059

Depositor

Randall Moon

Article

Ekker et al Curr Biol. 1995 Aug 1. 5(8):944-55.

Insert

sonic hedgehog (shha Zebrafish)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Expression

Mammalian

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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