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PB-TA-ERN

Plasmid

#80474

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)

Depositor

Knut Woltjen

Publication

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Publication

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Publication

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Tags

His6 tag

Expression

Bacterial

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Publication

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Tags

None

Expression

Mammalian

Promoter

CAG

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV3flag8HOIL-1L

Plasmid

#50016

Purpose

Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Publication

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGEX-GST-AGO2

Plasmid

#24317

Depositor

Carl Novina

Publication

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO2 (AGO2 Human)

Tags

GST

Expression

Bacterial

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pEX-GFP-hLC3WT

Plasmid

#24987

Depositor

Isei Tanida

Publication

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Tags

EGFP

Expression

Mammalian

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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p3xFLAG-CMV10-hAtg13

Plasmid

#22872

Depositor

Noboru Mizushima

Publication

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Publication

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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aMHC-puro rex-neo

Plasmid

#21230

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pStart-K

Plasmid

#20346

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Gateway Cloning and Mouse Targeting

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Murine cell surface protein knockout (McspKO) library

Pooled library

#200445

Purpose

Knockout library targeting mouse cell surface genes.

Depositor

Junjie Chen

Publication

Wang et al Proc Natl Acad Sci U S A. 2022 Jun 21;11

Expression

Mammalian

Species

Mus musculus

Use

CRISPR, Lentiviral, and Mouse Targeting

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only
pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Publication

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Tags

3XFLAG and 6XHis

Expression

Bacterial

Promoter

T7

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLB2 Ubi-FLIP FF

Plasmid

#19753

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, Lentiviral, Mouse Targeting, and RNAi

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM3

Plasmid

#58397

Purpose

Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Publication

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (IFITM3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Publication

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEiLOV-N1

Plasmid

#26588

Depositor

John Christie

Publication

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Expression

Mammalian

Available since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLCKO

Plasmid

#73311

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

Depositor

Jason Moffat

Publication

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pGWB533

Plasmid

#74872

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GUS (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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