We narrowed to 59,360 results for: ran

1. PCMV-intron myc Rab6 T27N

   Plasmid

   #46782

   Purpose

   expression of T27N Rab6a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab6 T27N (RAB6A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   T27N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. PCMV-intron myc Rab11 S25N

   Plasmid

   #46786

   Purpose

   expression of S25N Rab11a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB11A S25N (RAB11A Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. Rab8-T22N(Flag) in pcDNA3.1neo

   Plasmid

   #46784

   Purpose

   expression of T22N Rab8a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB8A T22N (RAB8A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T22N dominant negative

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

   Plasmid

   #52890

   Purpose

   "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

   Depositor

   Iswar Hariharan

   Article

   Bosch et al Development. 2015 Feb 1;142(3):597-606.

   Inserts

   LexGAD
   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Insect

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEos3.2FP

   Plasmid

   #203757

   Purpose

   Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   NRas c-term (NRAS Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEM-HSPA5P

   Plasmid

   #44150

   Purpose

   a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

   Depositor

   Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   HSPA5 promoter (HSPA5 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUDE710

   Plasmid

   #103020

   Purpose

   expression of a Cpf1 programming crRNA targeting ADE2 and HIS4 (crADE2-3 crHIS4-4.S)

   Depositor

   Jean-Marc Daran

   Article

   Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

   Insert

   crADE2-3.crHIS4-4.S (ADE2, HIS4 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   SNR52

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFRT-DHX36iso2E335A

   Plasmid

   #159588

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pF713-pEGFP-N1-Hs-FL-METTL20 wt

   Plasmid

   #85114

   Purpose

   transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available Since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1_ZKSCAN1_Tau7-12WT

    Plasmid

    #194166

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 7-12 WT
    

    Tags

    3X Flag

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1_ZKSCAN1_Tau10-12WT

    Plasmid

    #194163

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
    

    Tags

    3X flag tag

    Expression

    Mammalian

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ORAI1-YFP T184M

    Plasmid

    #114181

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M (ORAI1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

    Plasmid

    #30528

    Depositor

    Gerhart Ryffel

    Article

    Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Cre/Lox; Flp/frt

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P328L329delCCTCT

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1

    Plasmid

    #42189

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    Gamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K68A,K69A,F70A,F71A

    Promoter

    CMV

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

16. EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1

    Plasmid

    #42190

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    G protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A73L,L74I,L75S

    Promoter

    CMV

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pBiFC-SS-VC155-IRE1-dLD

    Plasmid

    #217751

    Purpose

    Expresses the VC fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    VC155-IRE1-dLD (ERN1 Human)
    

    Tags

    Venus (155-238, A206K)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-CD28(CD45TM)

    Plasmid

    #223626

    Purpose

    Lentiviral expression of human CD28 (CD45TM) in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Mutation

    CD28 transmembrane domain replaced by CD45 transm…

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid

    #203889

    Purpose

    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T16H2 promoter and 5'UTR
    

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUN1224 - pL0_pT3R (pro + 5U)

    Plasmid

    #203892

    Purpose

    T3R promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

    Depositor

    Carolyn Lee-Parsons

    Article

    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

    Insert

    T3R promoter and 5'UTR
    

    Expression

    Plant

    Mutation

    Mutation of BpiI or BsaI cut site for MoClo domes…

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge