We narrowed to 988 results for: Streptococcus

1. pDL278_P23-DsRed-Express2

   Plasmid

   #121505

   Purpose

   E. coli-streptococcal shuttle vector that expresses rfp

   Depositor

   Robert Shields

   Article

   Shields et al Appl Environ Microbiol. 2019 May 17. pii

   Insert

   DsRed-Express2
   

   Use

   E. coli streptococcal shuttle vector

   Promoter

   P23

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAC439

   Plasmid

   #195237

   Purpose

   Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAC565

   Plasmid

   #195242

   Purpose

   Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL0_12 [pAMβ1 ORI]

   Plasmid

   #198926

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAMβ1 ORI
   

   Use

   Synthetic Biology

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPM_Pveg-sfgfp

   Plasmid

   #121503

   Purpose

   Integration vector that expresses gfp in S. mutans

   Depositor

   Robert Shields

   Article

   Shields et al Appl Environ Microbiol. 2019 May 17. pii

   Insert

   Superfolder GFP
   

   Use

   S. mutans integration vector, integrates at the m…

   Promoter

   Pveg

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKIR1.1

   Plasmid

   #85758

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Plant

   Promoter

   AtRPS5A

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX165-HiFi Cas9

    Plasmid

    #140563

    Purpose

    Expresses HiFi Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Mutation

    R691A

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS448∙CsR

    Plasmid

    #122096

    Purpose

    CRISPR/Cas9 counterselection in Gram-negative bacteria

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pem7 and Pm

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAC569

    Plasmid

    #195241

    Purpose

    Mammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTdTomato-L5

    Plasmid

    #140994

    Purpose

    tdTomato, StrepR, L5int, attP to create RFP mycobacteria

    Depositor

    Wilbert Bitter

    Article

    Meijers et al Tuberculosis (Edinb). 2020 Sep;124:10198

    Insert

    tdTomato
    

    Expression

    Bacterial

    Promoter

    hsp60

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL1P1_AtU626Ter26

    Plasmid

    #191769

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL0_13 [pIP501 ORI]

    Plasmid

    #198927

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIP501 ORI
    

    Use

    Synthetic Biology

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pScI_dCas9-CDA

    Plasmid

    #108549

    Purpose

    Bacterial Target-AID vector

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Insert

    dCas9-PmCDA1
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    lambda OR

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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