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Plasmid#79597PurposeTransient and retroviral expressionDepositorInsertjunctate (ASPH Human)
UseRetroviralTagsFLAGExpressionMammalianMutation77DAD79 to 77AAA79PromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-MKII::nlsLexADBDo
Plasmid#64718Purposegenerating transgenic TRIC fliesDepositorInsertMKII::nlsLexADBDo
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat2-SH2
Plasmid#46512DepositorInsertStat2 (STAT2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 566-681PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX bTat (P#699)
Plasmid#14652DepositorInsertbTat
UseTagsExpressionBacterialMutationAmino acids 1-103PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLINK bTat (P#697)
Plasmid#14653DepositorInsertbTat
UseTagsmycExpressionMammalianMutationAmino acids 1-103PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-cytoFLARE1.0-TEV
Plasmid#234519PurposeTranscriptional reporter for detecting calcium transients in Drosophila larvae (TEVp)DepositorInsertCaM-V5-TEVp
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 1, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-Myc-rDMRdeltaTEAD
Plasmid#235695PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assayDepositorInsertMyc (Myc Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Cyr61-rDMRdeltaTEAD
Plasmid#235693PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assayDepositorInsertCyr61 (Ccn1 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Ctgf-rDMRdeltaTEAD
Plasmid#235697PurposeMeasure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assayDepositorInsertCtgf (Ccn2 Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL2-deltaTAR-luciferase
Plasmid#215896PurposeExpression of luciferaseDepositorInsertluciferase
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaT
Plasmid#216888PurposeMega' with knockout of GvpTDepositorInsertMega' with knockout of GvpT
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pST39-Mega'-deltaTU
Plasmid#216944PurposeMega' with knockout of GvpT and GvpUDepositorInsertMega' with knockout of GvpT and GvpU
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)
Plasmid#194217PurposeLentiviral transducition vector for a GAL4-responsive tagBFP reporter geneDepositorInsertUAS-ybTATA-tagBFP; PGK-mCitrine
UseLentiviralTagsExpressionMutationPromoterGAL4 UAS-ybTATA; PGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaORDdeltaTMD-FKBP
Plasmid#220074Purposeexpresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaORDdeltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaORDdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-ORP5deltaTMD-FKBP
Plasmid#220073Purposeexpresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.DepositorInsertOSBPL5deltaTMD (OSBPL5 Human)
UseTagsExpressionMammalianMutationdeltaTMDPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EhT
Plasmid#215546PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EPG
Plasmid#215545PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K49R-STAT3
Plasmid#203721PurposeExpresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K49R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K685R-STAT3
Plasmid#203722PurposeExpresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K685R substitutionsPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only