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pRK5-HA GST RagB 54L

Plasmid

#19302

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T54L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

Plasmid

#50942

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6s

Use

AAV

Promoter

hSyn1

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFYF1320 EGFP Site#1

Plasmid

#47511

Purpose

human gRNA expression vector targeting EGFP

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-EGFP site 1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-rab9 DN

Plasmid

#12664

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(H134R)-EYFP

Plasmid

#26969

Purpose

AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Histidine 134 is mutated to Arginine

Promoter

CaMKIIa

Available Since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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7M8

Plasmid

#64839

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

Insert

7M8 cap

Use

AAV

Mutation

7mer insertion in the AAV2 cap

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

S218D S222D

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Tags

EGFP and HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pHB9-EGFP (Hb9 promoter) [TJ#96]

Plasmid

#16275

Depositor

Thomas Jessell

Article

Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Insert

HB9 promoter

Use

Mouse Targeting

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-TRBP

Plasmid

#15666

Depositor

Dong-Yan Jin

Article

Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

Insert

TRBP (TARBP2 Human)

Expression

Mammalian

Available Since

Sept. 19, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Available Since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-hPIASx beta

Plasmid

#15210

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx beta (PIAS2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-LKB1

Plasmid

#21147

Depositor

Junying Yuan

Article

Karuman et al Mol Cell. 2001 Jun . 7(6):1307-19.

Insert

GFP-LKB1 (Stk11 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only
Flag-hPIASx alpha

Plasmid

#15209

Depositor

Ke Shuai

Article

Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

Insert

PIASx alpha (PIAS2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_GPHN.FingR-mKate2-IL2RGTC

Plasmid

#46297

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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