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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Publication

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Publication

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

83 Articles

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pRK1043

Plasmid

#8835

Depositor

David Waugh

Publication

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

tobacco etch virus protease, catalytic domain

Tags

MBP and polyarginine

Expression

Bacterial

Mutation

Autolysis-resistant S219V mutant

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDsRed-attP

Plasmid

#51019

Purpose

Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Inserts

attP
LoxP-3xP3-DsRed-LoxP

Use

High copy, amp resistance

Promoter

3xP3 and None

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

88 Articles

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LZRS-HOTAIR

Plasmid

#26110

Depositor

Howard Chang

Publication

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

HOTAIR (HOTAIR Human)

Use

Retroviral

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pDsRed-attP

Plasmid

#51019

Purpose

Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Publication

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Inserts

attP
LoxP-3xP3-DsRed-LoxP

Use

High copy, amp resistance

Promoter

3xP3 and None

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

88 Articles

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Publication

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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MSCV h c-MYC IRES GFP

Plasmid

#18119

Depositor

John Cleveland

Insert

c-MYC (MYC Human)

Use

Retroviral

Tags

GFP

Mutation

The CUG which is the start site for Myc I is pres…

Available since

June 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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LZRS-HOTAIR

Plasmid

#26110

Depositor

Howard Chang

Publication

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

HOTAIR (HOTAIR Human)

Use

Retroviral

Available since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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MSCV-XZ066-EGFRvIII

Plasmid

#20737

Depositor

Alonzo Ross

Publication

Li et al Neuro Oncol. 2009 Feb . 11(1):9-21.

Insert

EGFRvIII (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

EGFR with a 267 amino acid deletion in the extrac…

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Publication

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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MSCV h c-MYC IRES GFP

Plasmid

#18119

Depositor

John Cleveland

Insert

c-MYC (MYC Human)

Use

Retroviral

Tags

GFP

Mutation

The CUG which is the start site for Myc I is pres…

Available since

June 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Publication

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Publication

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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lentiCas9n(D10A)-Blast

Plasmid

#63593

Purpose

Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

Inserts

Cas9
Blasticidin resistance

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, nickase mutant

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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