We narrowed to 6,241 results for: cas9 expression plasmid

1. MAP3K2 gRNA (BRDN0001147901)

   Plasmid

   #75511

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MAP3K2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MAP3K2 (MAP3K2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ACVR2B gRNA (BRDN0001147208)

   Plasmid

   #75485

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ACVR2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ACVR2B (ACVR2B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ACVR2B gRNA (BRDN0001149036)

   Plasmid

   #75486

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ACVR2B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ACVR2B (ACVR2B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PDGFRA gRNA (BRDN0001145116)

   Plasmid

   #75491

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PDGFRA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PDGFRA gRNA (BRDN0001145903)

   Plasmid

   #75492

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PDGFRA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BCKDK gRNA (BRDN0001147891)

   Plasmid

   #75504

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BCKDK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BCKDK (BCKDK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BMPR1A gRNA (BRDN0001147849)

   Plasmid

   #77959

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BMPR1A

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   BMPR1A (BMPR1A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. IPMK gRNA (BRDN0001144988)

   Plasmid

   #78076

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human IPMK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   IPMK (IPMK Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FASTKD3 gRNA (BRDN0001146322)

   Plasmid

   #78063

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FASTKD3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FASTKD3 (FASTKD3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PTK7 gRNA (BRDN0001146728)

    Plasmid

    #78024

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PTK7

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PTK7 (PTK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BCR gRNA (BRDN0001148322)

    Plasmid

    #76908

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BCR

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    BCR (BCR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DLG1 gRNA (BRDN0001147622)

    Plasmid

    #76620

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DLG1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DLG1 (DLG1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CSNK1D gRNA (BRDN0001146126)

    Plasmid

    #76715

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CSNK1D

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CSNK1D (CSNK1D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AMHR2 gRNA (BRDN0001147673)

    Plasmid

    #76658

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AMHR2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AMHR2 (AMHR2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CHKA gRNA (BRDN0001145792)

    Plasmid

    #76586

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CHKA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CHKA (CHKA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AURKC gRNA (BRDN0001147545)

    Plasmid

    #78053

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AURKC

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AURKC (AURKC Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IP6K2 gRNA (BRDN0001146075)

    Plasmid

    #77816

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K2 (IP6K2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CDC42BPB gRNA (BRDN0001147279)

    Plasmid

    #77779

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDC42BPB

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDC42BPB (CDC42BPB Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACVRL1 gRNA (BRDN0001147340)

    Plasmid

    #77783

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ACVRL1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ACVRL1 (ACVRL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLK4 gRNA (BRDN0001145430)

    Plasmid

    #77797

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CLK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CLK4 (CLK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CLK4 gRNA (BRDN0001146113)

    Plasmid

    #77798

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CLK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CLK4 (CLK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ARSG gRNA (BRDN0001147989)

    Plasmid

    #77640

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ARSG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ARSG (ARSG Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GUCY2F gRNA (BRDN0001147943)

    Plasmid

    #77254

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GUCY2F

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    GUCY2F (GUCY2F Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CAMK4 gRNA (BRDN0001145862)

    Plasmid

    #77226

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CAMK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CAMK4 (CAMK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ANKK1 gRNA (BRDN0001149290)

    Plasmid

    #76035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ANKK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ANKK1 (ANKK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LX-miR

    Pooled Library

    #112200

    Purpose

    gRNA library targets human primary miRNAs

    Depositor

    Ren-Jang Lin

    Article

    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    Lentiviral

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

27. pCG-scr

    Plasmid

    #229847

    Purpose

    Expresses wild-type Cas9 and scrambled gRNA.

    Depositor

    Natsuko Chiba

    Insert

    guide RNA with scrambled sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT040

    Plasmid

    #67640

    Purpose

    URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Insert

    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIK208

    Plasmid

    #65630

    Purpose

    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIK137

    Plasmid

    #65632

    Purpose

    Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

    Depositor

    Iskra Katic

    Article

    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

    Insert

    Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AspFlex Kit

    Plasmid Kit

    #1000000217

    Purpose

    This collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.

    Depositor

    Veronica Godoy-Carter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

33. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV MCS mCherry-KASH

    Plasmid

    #139654

    Purpose

    Cloning template for AAV-based ORANGE knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pYPQ210

    Plasmid

    #109329

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSC10

    Plasmid

    #104784

    Purpose

    pAH595-gRNA 2xplex entry cassette

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p1977 (pUC19-PTDH3-PTEF1)

    Plasmid

    #78230

    Purpose

    Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    Bidirectional promoters pTDH3-pTEF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

40. SGL40C-H1.EFS.RFP657

    Plasmid

    #69148

    Purpose

    Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge