167,318 results

1. mTagBFP2-Lifeact-7

   Plasmid

   #54602

   Purpose

   Localization: Actin, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Type

   Empty backbone

   Tags

   Lifeact and mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV GFP DEST (736-1)

   Plasmid

   #19732

   Purpose

   3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Gateway destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. p4xmts-mScarlet3_N1

   Plasmid

   #189774

   Purpose

   Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   4xmts-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TBG-ER-TurboID

   Plasmid

   #149415

   Purpose

   Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   TBG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetOne-mCherry-cNLS

   Plasmid

   #138527

   Purpose

   Inducible expression of mCherry with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   mCherry
   

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE3Gs

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmScarlet3-Giantin_C1

   Plasmid

   #189773

   Purpose

   Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-Giantin
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-His-hUbi

   Plasmid

   #31815

   Depositor

   Astar Winoto

   Article

   Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

   Insert

   Ubiquitin (UBB Human)
   

   Tags

   HIS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. nuclear cpVenus control

    Plasmid

    #186790

    Purpose

    Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSG5-BZLF1

    Plasmid

    #72637

    Purpose

    Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BZLF1
    

    Expression

    Mammalian

    Mutation

    Q163R

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCAD29_hIRG1_4-461_pvp008

    Plasmid

    #124843

    Purpose

    Expresses human CAD (Irg1) in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 462-end.

    Promoter

    T7

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUtdTW

    Plasmid

    #22478

    Depositor

    Connie Cepko

    Article

    Rompani et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    tandem dimer tomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.3_SARS2_JN.1

    Plasmid

    #218678

    Purpose

    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (JN.1)
    

    Expression

    Mammalian

    Mutation

    L455S on the basis of BA.2.86

    Promoter

    CMV

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-S5E2-dTom-nlsdTom (AAV PHP.eB)

    Viral Prep

    #135630-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Tags

    dTomato

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CAG-GFP

    Plasmid

    #37825

    Depositor

    Edward Boyden

    Has Service

    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-HSF1

    Plasmid

    #32537

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMevT

    Plasmid

    #17815

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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