We narrowed to 6,093 results for: pcDNA 3.1

1. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

   Plasmid

   #50898

   Purpose

   Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   G719C C723S C724V in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

   Plasmid

   #111462

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

   Plasmid

   #111497

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176540

   Purpose

   Mammalian expression of nuclear localized OgeuIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   OgeuIscB
   

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

   Plasmid

   #176538

   Purpose

   Mammalian expression of nuclear localized AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

   Plasmid

   #166834

   Purpose

   Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

   Plasmid

   #228400

   Purpose

   Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

   Depositor

   Dirk Daelemans

   Article

   Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

   Insert

   NES/PKI-mNeonGreen2x-NLS/FUS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182682

   Purpose

   Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Insert

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   

   Expression

   Mammalian

   Promoter

   NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

   Plasmid

   #182685

   Purpose

   Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV and NA

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion (NT96)

    Plasmid

    #50914

    Purpose

    Expresses human NKCC1 with truncation of N-terminus and mutations at P676C I730C. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C N-term deletion of aa13-222 in hNKCC1…

    Promoter

    CMV

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 Flag YFP hNKCC1 A675C C723S C724V A735C (NT847)

    Plasmid

    #50865

    Purpose

    Expresses human NKCC1 A675C C723S C724V A735C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A675C C723S C724V A735C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid

    #50913

    Purpose

    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S150N S155Q S170Q T177A S183R S193E T203A T205A T…

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(TAG)-3xFLAG donor

    Plasmid

    #182686

    Purpose

    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linkerA6TAG-3xFLAG. Can be used as a donor for the tagging of endogenous NFL via TKIT approach and click labeling of NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse Nefl intron3-exon4-linker(TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Expression

    Mammalian

    Promoter

    NA

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid

    #50912

    Purpose

    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C and I730C; N-term deletion of aa13-222; C-t…

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-HisFlag-rUSP19CSdomains

    Plasmid

    #155240

    Purpose

    for mammalian overexpression of the CS domains of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (CS domains) (Usp19 Rat)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-HisFlag-rUSP19CYT

    Plasmid

    #155238

    Purpose

    for mammalian overexpression of the cytoplasmic isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (Cytoplasmic isoform) (Usp19 Rat, Mouse)
    

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcmCherry 3.1(-)

    Plasmid

    #62803

    Purpose

    Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry

    Depositor

    Michal Minczuk

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-HA FoxP3

    Plasmid

    #229615

    Purpose

    Mammalian expression of full length mouse FoxP3

    Depositor

    Sun Hur

    Insert

    full length FoxP3 (Foxp3 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid

    #69058

    Purpose

    Dual Fluorescent Reporter for F-actin and Nuclei

    Depositor

    Olivier Pertz

    Insert

    LifeAct-GFP_IRES_NLS-mCherry
    

    Tags

    Lifeact-GFP, NLS-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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