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SPACE-NG (pRZ5150)

Plasmid

#140244

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_NG

Expression

Mammalian

Mutation

V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

Plasmid

#155306

Purpose

Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA BFP

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f

Plasmid

#72304

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CaMKIIa

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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EBFP2-Nucleus-7

Plasmid

#55249

Purpose

Localization: Nuclear Localization, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

SV40 T-Antigen Nuclear Localization Signal (NLS)

Tags

EBFP2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-Keratin-17

Plasmid

#55065

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Keratin (KRT18 Human)

Tags

mCherry

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
EF1a_CDX2_P2A_Hygro_Barcode

Plasmid

#120432

Purpose

Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

CDX2 (CDX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-PRM-B0034-geneVI

Plasmid

#80858

Purpose

Accessory plasmid (encodes M13 gene VI)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Insert

M13 gene VI

Expression

Bacterial

Promoter

Lambda Prm

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pR0306 CcaCas13b His6-TwinStrep-SUMO

Plasmid

#182687

Purpose

Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

CcaCas13b

Tags

SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1662

Plasmid

#112050

Purpose

C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Inserts

IAA17
TIR1

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

ADH1 and None

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Article

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSKDuet16

Plasmid

#41684

Depositor

Hideo Iwai

Article

Ellila et al FEBS Lett. 2011 Oct 10.

Insert

NpuDnaE intein

Tags

GB1 and His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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CTV

Plasmid

#27803

Depositor

Steven Vogel

Article

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Insert

Cerulean Traf Venus

Use

Fret standard

Expression

Mammalian

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-MCP-huADARE488QT490A

Plasmid

#159922

Purpose

Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

Human ADAR2 E488QT490A (ADARB1 Human)

Use

AAV

Promoter

EF1a

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

Plasmid

#156438

Purpose

For transient expression of mouse CTCF tagged with eGFP

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse CTCF (Ctcf Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p006-GFP-pBAD

Plasmid

#108315

Purpose

GFP from jellyfish Aquorea sp. under pBAD promoter with AraC repressor; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

GFP under control of arabinose-inducible promoter pBAD

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA-2A-NptII_ETR

Plasmid

#91695

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtr

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Integrin-Alpha2-N-18

Plasmid

#54128

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Integrin-Alpha2 (ITGA2 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits