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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Publication

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pTP1172

Plasmid

#104161

Purpose

Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

Depositor

Dirk Görlich

Publication

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Syn-Chronos-GFP (AAV5)

Viral prep

#59170-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Syn-driven Chronos-GFP expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

Syn

Tags

GFP

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only
pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Publication

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Publication

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Tags

sfCherry11x4

Expression

Mammalian

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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NLS-mCherry-LEXY (pDN122)

Plasmid

#72655

Purpose

Blue light-inducible nuclear export of mCherry

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-LEXY

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Publication

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Publication

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pmTurquoise2-Peroxi

Plasmid

#36203

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

Peroxisomal targeting sequence

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-HA (New MCS)

Plasmid

#32530

Depositor

Christopher A Walsh

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA3 GFP LIC cloning vector (6D)

Plasmid

#30127

Depositor

Scott Gradia

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Flag-HA-BAP1

Plasmid

#22539

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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Thy1 promoter construct

Plasmid

#20736

Depositor

Joshua Sanes

Publication

Feng et al Neuron. 2000 Oct . 28(1):41-51.

Insert

Thy1 promoter (Thy1 Mouse)

Expression

Mammalian

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCI-neo beta catenin WT

Plasmid

#16518

Depositor

Bert Vogelstein

Publication

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pcDNA3-EGFP-Rac1(Q61L)

Plasmid

#13720

Depositor

Klaus Hahn

Publication

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(Q61L) (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pBABE-FLAG-KD LKB1

Plasmid

#8593

Depositor

Lewis Cantley

Publication

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K78I: lysine 78 mutated to isoleucine; kinase dead

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pCW57-CMV-ssRFP-GFP-KDEL

Plasmid

#128257

Purpose

Fluorescence reporter to monitor ER-phagy activity

Depositor

Noboru Mizushima

Publication

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

ssRFPGFPKDEL

Use

Lentiviral

Tags

RFPGFP

Expression

Mammalian

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Publication

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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Membrane protein gRNA library

Pooled library

#113345

Purpose

Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform.

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Species

Homo sapiens

Use

Lentiviral

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles