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We narrowed to 152,655 results for: des

Showing: 4881 - 4920 of 152655 results

  1. pVH028

    Plasmid
    #80393
    Purpose
    Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Taz chimeric histine kinase
    CusS (cusS )
    NahR (nahR )
    Use
    Synthetic Biology
    Tags
    Fused by GS linkers to 4 SH3 domains
    Expression
    Bacterial
    Mutation
    Glycine 448 changed to Alanine, makes it primaril…
    Promoter
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    80393-plasmid-map-sequence-id-166533

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  5. pMSP1D1T1

    Plasmid
    #97327
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of T residue after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97327-plasmid-map-sequence-id-182625

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  6. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

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  9. pcDNA3.1(+)MBII-85-3'ss mut

    Plasmid
    #67644
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.
    Depositor
    Stefan Stamm
    Article
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    3' splice site muattion
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67644-plasmid-map-sequence-id-116911

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  10. pML2(+)BR

    Plasmid
    #108336
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108336-plasmid-map-sequence-id-214578

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  11. pML2(+)WF

    Plasmid
    #108333
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108333-plasmid-map-sequence-id-209522

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  12. pML2(+)BF

    Plasmid
    #108334
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108334-plasmid-map-sequence-id-209526

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  13. pML2(+)WR

    Plasmid
    #108335
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108335-plasmid-map-sequence-id-209243

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  14. pML2(−)BF

    Plasmid
    #108338
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108338-plasmid-map-sequence-id-209524

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  15. pML2(−)WR

    Plasmid
    #108339
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108339-plasmid-map-sequence-id-209534

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  16. pML2(−)BR

    Plasmid
    #108340
    Purpose
    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.
    Depositor
    Andrew Muscroft-Taylor
    Article
    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108340-plasmid-map-sequence-id-209245

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  17. pGDP3 aph(3'')-Ia

    Plasmid
    #112879
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Pbla
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112879-plasmid-map-sequence-id-219874

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  27. (P6f)3(P14f)3-gs

    Plasmid
    #228082
    Purpose
    Mammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    P6f, P14f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    228082-plasmid-map-sequence-id-454673

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  28. (S1h-S3h)3-gs

    Plasmid
    #228078
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    228078-plasmid-map-sequence-id-457750

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  31. TagBFP(S2h)8-gs

    Plasmid
    #228086
    Purpose
    Mammalian expression of coiled coil segments (S2h)x8, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228086-plasmid-map-sequence-id-454692

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  32. RFP-PPPPPPAAAA-gs

    Plasmid
    #228092
    Purpose
    Mammalian expression of 10 coiled coil segments in PPPPPPAAAA arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, PPPPPPAAAA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    228092-plasmid-map-sequence-id-451390

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  34. mCit(S1h)8-gs

    Plasmid
    #228084
    Purpose
    Mammalian expression of coiled coil segments (S1h)x8, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228084-plasmid-map-sequence-id-451170

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  35. TagBFP(S2h)12-gs

    Plasmid
    #228087
    Purpose
    Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228087-plasmid-map-sequence-id-451132

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  37. pGADT7-AP-2 σ2

    Plasmid
    #198345
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-2 σ2
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    R42G substitution, and silent substitutions in co…
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198345-plasmid-map-sequence-id-387832

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  38. pGADT7-AP-1 σ1B

    Plasmid
    #198335
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1B
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198335-plasmid-map-sequence-id-387734

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  39. pGADT7-AP-1 σ1A

    Plasmid
    #198334
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.
    Insert
    AP-1 σ1A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Expresses a LELQMNRRY nonapeptide after the last …
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198334-plasmid-map-sequence-id-387458

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Showing: 4881 - 4920 of 152655 results