We narrowed to 56,468 results for: CaS;

1. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

   Plasmid

   #159913

   Purpose

   Mutagenesis of Kcnj6

   Depositor

   Larry Zweifel

   Insert

   Kcnj6 (Kcnj6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSDest2-SpCas9-NLS-3XHA-NLS

   Plasmid

   #69220

   Purpose

   Expresses wild type SpCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti SYN-SVI-dCas9-VPR

   Plasmid

   #164575

   Purpose

   Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

   Depositor

   Jeremy Day

   Article

   Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

   Insert

   FLAG-dCas9-VPR containing an SV40 intron
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-wtSpCas9 (without sgRNA)

   Plasmid

   #92353

   Purpose

   Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-wild-type SpCas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mesp-1916/-1>nls::Cas9::nls

   Plasmid

   #59988

   Purpose

   Mesp driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Mesp -1916/-1

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. RCAS(A) human p110 alpha E545K

   Plasmid

   #52211

   Purpose

   expresses human p110 alpha with E545K mutation in avian cells

   Depositor

   Peter Vogt

   Article

   Kang et al Proc Natl Acad Sci U S A. 2005 Jan 18;10

   Insert

   p110 alpha E545K (PIK3CA Human)
   

   Use

   Retroviral; Avian

   Tags

   Expression

   Mutation

   Glutamic Acid 545 to Lysine

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

   Plasmid

   #101109

   Purpose

   Encodes dCas9(N)-DHFR fused to BDKRB2

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   BDKRB2-dCas9(N)-DHFR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Px458-Cas9n-Trp73-Exon4-3sgRNA

   Plasmid

   #88850

   Purpose

   CRISPR KO of Trp73

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp73 (Trp73 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Px458-Cas9n-Trp73-Exon4-4sgRNA

    Plasmid

    #88851

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73 (Trp73 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMT Laccase2 MCS-HIPK3 Exon

    Plasmid

    #69888

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Human, Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. RCAS (B) flag Lunatic Fringe (CT#471)

    Plasmid

    #13980

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. RCAS (A) flag Lunatic Fringe (CT#479)

    Plasmid

    #13979

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Lunatic Fringe (LFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid

    #160221

    Purpose

    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtACT2::Cas9-nls::tSlATPase
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-T Easy-Cas9-nls-CDS1

    Plasmid

    #160231

    Purpose

    Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
    

    Use

    Level 0 of moclo golden gate position cds1

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

    Plasmid

    #161924

    Purpose

    To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    sgRNA targeting RDH11 exon 2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)-ABL1-gRNA1-exon4

    Plasmid

    #163322

    Purpose

    eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 1_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

19. eSpCas9(1.1)-ABL1-gRNA2-exon4

    Plasmid

    #163323

    Purpose

    eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

    Depositor

    Val Brunton

    Article

    Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

    Insert

    ABL1 gRNA 2_Exon4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

20. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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