We narrowed to 11,615 results for: VARS

1. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

   Plasmid

   #175536

   Purpose

   Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORF1-2

   Plasmid

   #206024

   Purpose

   An expression vector for mouse LINE1 ORF1-2

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   ORF1-2 derived from mouse LINE1
   

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GeNL(Ca2+)_480/pcDNA3

   Plasmid

   #85205

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_480
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. epB_UbC_CymRV5-nls-Halox2_DEx4

   Plasmid

   #119907

   Purpose

   ePiggyBac mammalian expression plasmid for CymR-Halo fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   CymRV5-nls-Halox2
   

   Expression

   Mammalian

   Available Since

   April 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_dCas9-1x7

   Plasmid

   #63601

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Expression

   Mammalian

   Promoter

   U6, miniCMV

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mTFP1-C1

   Plasmid

   #54613

   Purpose

   Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Tags

   mTFP1

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCF4 in pENTR

   Plasmid

   #216569

   Purpose

   gateway cloning donor vector containing TCF4

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE-RA2.0

    Plasmid

    #208299

    Purpose

    Expresses ABE-RA2.0 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA2.0-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    P48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE-RA3.1

    Plasmid

    #208302

    Purpose

    Expresses ABE-RA3.1 in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA-RA3.1-SpCas9 D10A
    

    Expression

    Mammalian

    Mutation

    E27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_HaloTag9_SKL

    Plasmid

    #175542

    Purpose

    Peroxysomal expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-SKL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTiger-OptoSOS

    Plasmid

    #86439

    Purpose

    Single-construct blue light optogenetic Ras activator for use in Drosophila

    Depositor

    Jared Toettcher

    Article

    Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

    Insert

    OptoSOS
    

    Tags

    tagRFP

    Expression

    Insect

    Promoter

    Gal4-UAS

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-oChIEF-citrine

    Plasmid

    #50974

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW211b5

    Plasmid

    #136927

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9(N)-NpuN
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    see manuscript

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST-K

    Plasmid

    #44560

    Purpose

    for ectopic constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX165-LZ3 Cas9

    Plasmid

    #140561

    Purpose

    Expresses LZ3 Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Mutation

    N690C T769I G915M N980K

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST-KiT

    Plasmid

    #44562

    Purpose

    for integrative inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-oChIEF-tdTomato

    Plasmid

    #50977

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-30nmSAH-DmrA

    Plasmid

    #61680

    Purpose

    GFP-labeled SAH scaffold with DmrA attachment site

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    mEGFP-30nmSAH-DmrA
    

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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