We narrowed to 11,276 results for: Vars

1. pLenti-TagGFP2

   Plasmid

   #121426

   Purpose

   TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

   Depositor

   Stanley Qi, Ovijit Chaudhuri

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   empty TagGFP2
   

   Use

   Lentiviral

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-Cas9

   Plasmid

   #64237

   Purpose

   expresses codon optimized Cas9 in zebrafish

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GeNL(Ca2+)_520/pcDNA3

   Plasmid

   #85204

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_520
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

   Plasmid

   #175536

   Purpose

   Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Beta4Gal-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSK-Sasg

   Plasmid

   #214350

   Purpose

   Expresses cloning backbone for SlugCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-oChIEF-tdTomato

   Plasmid

   #50977

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_b4g-HaloTag9

    Plasmid

    #175546

    Purpose

    Expression of HaloTag9 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW199b2

    Plasmid

    #136928

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gIII
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GeNL(Ca2+)_60/pcDNA3

    Plasmid

    #85207

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Endo-APEX2

    Plasmid

    #215553

    Purpose

    Stable, inducible expression in endosome

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    EGFP-2xFYVE-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. DDX3X in pENTR

    Plasmid

    #216572

    Purpose

    gateway cloning donor vector containing DDX3X

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SaABEmax

    Plasmid

    #119814

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. epB_CAG_TetRFlag-nls-tdTom_DEx4

    Plasmid

    #119909

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-tdTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge