We narrowed to 58,498 results for: plasmid
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Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDH5-AbR-GFP
Plasmid#122970PurposeLenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.DepositorInsertsCDH5p
CopGFP
Zeocin
UseLentiviralExpressionMammalianMutationNAAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSynapsin-PE2-Cterminal-lenti
Plasmid#135956PurposeNeuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) C-terminal
UseLentiviralExpressionMammalianMutationMMLV RT(D200N, T306K, W313F, T330P, L603W)PromoterhSynapsinAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-IPP1-His
Plasmid#118978PurposeBacterial expression plasmid for Inorganic pyrophosphataseDepositorInsertinorganic diphosphatase IPP1 (IPP1 Budding Yeast)
Tags6x-HisExpressionBacterialPromoterT7 promoterAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEff
Plasmid#97423PurposeTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Lb
Plasmid#86197PurposeLbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRPromoterMaize ubiquitin 1Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-YFP-STM2(15-746)
Plasmid#18862DepositorInsertStromal interaction molecule 2 (STIM2 Human)
TagsYFPExpressionMammalianMutationYFP inserted between signal peptide(SP) of STIM1 …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-M2
Plasmid#135584PurposeHigh eukaryotic dual protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorExpressionInsectPromoterpolyhedrin of AcMNPVAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PE2max-BSD
Plasmid#191102PurposeLentiviral expression plasmid of PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
UseCRISPR and LentiviralExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9PromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)
Viral Prep#51669-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (AAV9)
Viral Prep#162382-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE (#162382). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of the ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001148862)
Plasmid#80263Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickExpressionBacterialMutationR364S mutation in MKPromoterPlacUV5Available SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium OPEN Accessory Reagent Set
Plasmid Kit#1000000013PurposePlasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method.DepositorApplicationGenome EditingVector TypeBacterial Expression, Insect Expression, Mammalian Expression, Plant ExpressionEditing TypeZFNAvailable SinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRMPVIR
Plasmid#27994PurposeRetroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.DepositorInsertTRMPVIR (shRen)
UseRNAi and Retroviral; Fluorescent reportersExpressionMammalianAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-centrin-1
Plasmid#72641PurposeThe plasmid encodes centrin-1 with an N-terminal EGFP tagDepositorAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)
Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralPromoterEF1 alphaAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-EYFP (AAV PHP.eB)
Viral Prep#26973-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFPAvailable SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only