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pJBL7010

Plasmid

#136942

Purpose

Constitutive sfGFP expression (high), bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLG026 (tmn)

Plasmid

#157904

Purpose

Expresses the tmn defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

tmn (transmembrane ATPase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE46

Plasmid

#90384

Purpose

NFYA - constitutive enhancer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NF-Y

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-sgRNA-v2

Plasmid

#204639

Purpose

Expression of trancated AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

trancated AsCas12f sgRNA (sgRNA-v2)

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

Plasmid

#34911

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE54

Plasmid

#90399

Purpose

C/EBPa - CEBPA gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

CEBPa

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-NLS-TurboID

Plasmid

#163849

Purpose

In vitro transcription of nls-TurboID. Parton lab clone JST

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

nls

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO3

Plasmid

#53143

Purpose

E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO3 (SUMO2 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-93)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMCSG7-Wt-NmeCas9

Plasmid

#71474

Purpose

bacterial pMCSG7 expression vector expressing Wildtype Nme cas9 with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-WT-NmeCas9

Tags

6His

Expression

Bacterial

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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PAGFP-Ub

Plasmid

#11933

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (RBX1 Human)

Tags

PAGFP

Expression

Mammalian

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE39

Plasmid

#90379

Purpose

PPARg - PPARg homodimer gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

PPARg

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLG031 (upx)

Plasmid

#157909

Purpose

Expresses the upx defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

upx (putative PD-(D/E)xK family phosphodiesterase)

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPAdGO2-PGK-Neo

Plasmid

#136899

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO2

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TAG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE42

Plasmid

#90380

Purpose

GATA3 - Gata3 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

GATA3

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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CE-8e-SpRY

Plasmid

#188913

Purpose

A-to-G base editor

Depositor

Xu Ma

Article

Cao et al Mol Ther Nucleic Acids. 2022 May 4;28:73

Insert

nSpRYCas9 ecTadA-8e

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-L

Plasmid

#234657

Purpose

Expresses L gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV L gene (L Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-P

Plasmid

#234656

Purpose

Expresses P gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV P gene (Nvgp2 Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTM-NiV-N

Plasmid

#234655

Purpose

Expresses N gene of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Insert

NiV N gene (N Nipah virus)

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1180-NiV L(2k)-mCherry/eGFP

Plasmid

#234654

Purpose

Minigenome plasmid of NiV Bangladesh strain

Depositor

Carolina Lopez

Article

Li et al J Virol. 2024 Jun 13;98(6):e0050324. doi

Inserts

part L gene
mCherry
eGFP

Use

In cell lines expressing bacteriophage t7 rna pol…

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVhTRIM65_3Flag

Plasmid

#53966

Purpose

Human TRIM65 mammalian expression vector with 3xFLAG epitope at c-terminus.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

TRIM65 (TRIM65 Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pLG024 (SIR2 + HerA)

Plasmid

#157902

Purpose

Expresses the SIR2 + HerA defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

SIR2 + HerA

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLX208-FLiCRE2-TF

Plasmid

#234523

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

CD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-SauCas9

Plasmid

#174040

Purpose

In vitro transcription of SauCas9 mRNA

Depositor

James Gagnon

Article

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Insert

SauCas9

Use

In vitro transcription

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1

Plasmid

#67900

Purpose

Expresses a fusion of mCherry with FKBP12 in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET14-Encap

Plasmid

#86405

Purpose

Expresses T. maritima encapsulin in E. coli

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Insert

Encapsulin

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLG002 (BREX type I)

Plasmid

#157880

Purpose

Expresses the BREX type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

BREX type I

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-E1103G

Plasmid

#10989

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1103G (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3308 A to G in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pLG003 (Druantia type I)

Plasmid

#157881

Purpose

Expresses the Druantia type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Druantia type I

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE12

Plasmid

#90347

Purpose

FXR - Farnesoid X receptor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

FXR

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG365-redFAST

Plasmid

#160354

Purpose

Expresses redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG008 (Ec78 retron)

Plasmid

#157886

Purpose

Expresses the Ec78 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec78 retron

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGE-T7

Plasmid

#153021

Purpose

Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

Depositor

Vito Valiante

Article

Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-NeonGreen

Plasmid

#139364

Purpose

FRET Standard

Depositor

Richard Day

Article

Reissaus et al Biophys J. 2020 Apr 21;118(8):1820-1829.

Insert

Scarlet 10AA Neon green

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBB-XIAPmut

Plasmid

#11559

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Tags

Flag

Expression

Mammalian

Mutation

changed his 467 to Alanine

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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