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pAN-PA1-RFP

Plasmid

#62247

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA1

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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U6>Ebf.774(F+E)

Plasmid

#59990

Purpose

U6 promoter driving Ebf.774 (F+E) sgRNA

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

Ebf.774 (F+E) sgRNA

Use

CRISPR

Promoter

Ciinte.U6

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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XE47 dnWnt-8

Plasmid

#16858

Depositor

Randall Moon

Article

Hoppler et al Genes Dev. 1996 Nov 1. 10(21):2805-17.

Insert

dnWnt-8 (wnt8a.L Frog)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-DN

Plasmid

#8502

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Tags

His

Expression

Bacterial

Mutation

Dominant Negative, C114S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pET28CLY4

Plasmid

#21763

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 4 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pDCAF-ABC

Plasmid

#113652

Purpose

Demethylates methylxanthines.

Depositor

Jeffrey Barrick

Article

Gutierrez et al Appl Environ Microbiol. 2019 Nov 14;85(2

Insert

ndmA and ndmB and ndmC

Use

Synthetic Biology

Available Since

Nov. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pFOSGE_COF4A01

Plasmid

#71016

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Msh6 (Msh6 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PTet-YFP

Plasmid

#62245

Purpose

used to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

YFP

Expression

Bacterial

Promoter

Ptet

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCG34

Plasmid

#26940

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

cgh-1 (cgh-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

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1xTRE DREaMR 3p3GFP

Plasmid

#182203

Purpose

1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-GFP

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET20b-pIgR D1-H6

Plasmid

#12530

Depositor

Pamela Bjorkman

Article

Hamburger et al Structure. 2004 Nov . 12(11):1925-35.

Insert

human polymeric immunoglobulin receptor (PIGR Human)

Tags

His

Expression

Bacterial

Mutation

Lys 1-Val 109

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pQLinkHD_mTFP1

Plasmid

#118858

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSGE_768_Nhe2-EcR_dCP

Plasmid

#71223

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

768_Nhe2-EcR_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_769_DipB-EcR_dCP

Plasmid

#71222

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

769_DipB-EcR_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+8NmCherry-ABCC1

Plasmid

#34942

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pLLU2G-Oct3

Plasmid

#21616

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pAN-AND

Plasmid

#62308

Purpose

synthetic circuit: AND gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A1NT sgRNA
A4NT sgRNA
A2NT sgRNA
A1NT

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, pA4, and pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_MC19_Trl-2xUAS_dCP

Plasmid

#71170

Purpose

Luciferase reporter

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MC19_Trl-2xUAS_dCP

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

DSCP Drosophila synthetic core promoter

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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