We narrowed to 3,461 results for: mpo

1. p2703-AGER-gRNA2

   Plasmid

   #216472

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2704-AGER-gRNA3

   Plasmid

   #216473

   Purpose

   to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   sgRNA targeting human AGER locus
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p2705-AGER-eGFP

   Plasmid

   #216474

   Purpose

   donor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   eGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

   Plasmid

   #216477

   Purpose

   lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CCM2_d410-444_eGFP

   Plasmid

   #208877

   Purpose

   Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids deletion : 410-444

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQCXIH FRB-BFP

   Plasmid

   #202425

   Purpose

   TagBFP FKBP-rapamycin binding (FRB) dimerization domain

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   FKBP-rapamycin binding (FRB) dimerization domain
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-Teton-puro-shFDPS #2

   Plasmid

   #198760

   Purpose

   conditional knockdown of FDPS

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

   Insert

   shFDPS #2 (FDPS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1-Teton-puro-shFDPS #1

   Plasmid

   #198759

   Purpose

   conditional knockdown of FDPS

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

   Insert

   shFDPS #1 (FDPS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pROS_phleo-Tps1/Gsy2

   Plasmid

   #196612

   Purpose

   Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-lambdaN-HA-DmTral-ADF_E

    Plasmid

    #146206

    Purpose

    Insect Expression of DmTral-ADF

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-ADF (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent mutations A198C, A387G, A1437G and 4…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmTral-FAF_E

    Plasmid

    #146207

    Purpose

    Insect Expression of DmTral-FAF

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-FAF (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent mutations A198C, A387G, A1437G and 4…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-lambdaN-HA-DmTral-FDA_E

    Plasmid

    #146208

    Purpose

    Insect Expression of DmTral-FDA

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-FDA (tral Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    three silent mutations A198C, A387G, A1437G and 4…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_Tau Authentic Alu 10-12WT

    Plasmid

    #194167

    Purpose

    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 WT
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1_Tau Authentic Alu 7-12WT

    Plasmid

    #194170

    Purpose

    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    microtubule-associated protein tau 7-12 WT
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_ZKSCAN1_Tau10-12V337M

    Plasmid

    #194165

    Purpose

    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 V337M
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Valine 337 to Methinonine

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eSpCas9-EF-5Flnc

    Plasmid

    #174870

    Purpose

    CRISPR vector for generating Flnc STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Flnc (Flnc Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

    Plasmid

    #182126

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

    Plasmid

    #182121

    Purpose

    pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    1N/2CxNLS-cMyc-NLP-cMyc AspCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge