We narrowed to 11,996 results for: pcDNA

1. pcDNA3-RAB-EKARev(T/A)

   Plasmid

   #118475

   Purpose

   Negative-control mutant for RAB-EKARev biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-Beclin1 1-242

   Plasmid

   #24391

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-242.

   Promoter

   Available since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 3xF-NHP2

   Plasmid

   #126871

   Purpose

   Expresses 3xFlag NHP2

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   NHP2 (NHP2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ExRai-AktAR(T/A)

   Plasmid

   #118463

   Purpose

   Negative-control mutant for ExRai-AktAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AktAR(T/A)
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3 Flag Jnk1a2

   Plasmid

   #13751

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1a2 (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3-hTLR3-VenusFlag

   Plasmid

   #197636

   Purpose

   Human CD283 protein expression in mammalian cells

   Depositor

   Haian Fu

   Insert

   TLR3 (TLR3 Human)
   

   Use

   Tags

   Venus-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CBX1mut-PA-mCherry

   Plasmid

   #138251

   Purpose

   Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

   Depositor

   Jin Zhang

   Article

   Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

   Insert

   CBX1mut-PAmCherry
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY111 (pcDNA3.1-huTsCpf1)

   Plasmid

   #92267

   Purpose

   Expression of humanized TsCpf1

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5/FRT-mMAPT

    Plasmid

    #231584

    Purpose

    Minigene of mouse MAPT containing exon 9, 10, 11 and relevant intronic sequences

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Cell Genom. 2024 Jun 12;4(6):100563. doi

    Insert

    mouse MAPT (Mapt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-DAKAP-μDpTT

    Plasmid

    #182324

    Purpose

    Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

    Depositor

    Jin Zhang

    Article

    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

    Insert

    DAKAP-μDronpa-TagRFP-T
    

    Use

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Mutation

    Dronpa contains H193T mutation.

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3/Myc-DNMT3B2

    Plasmid

    #36942

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B2 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT-TO-FH-Nsp16

    Plasmid

    #157724

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA5/FRT/TO V5 HSPA14

    Plasmid

    #19515

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 14 (HSPA14 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-FBL6-FLAG3C

    Plasmid

    #19904

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    FBL6 (FBXL6 Human)
    

    Use

    Tags

    FLAG3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3492 pcDNA3 Bad

    Plasmid

    #8778

    Depositor

    Stanley Korsmeyer

    Article

    Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

    Insert

    Bad (Bad Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-Mito-CaNAR2

    Plasmid

    #64731

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 30 amino acids from DAKAP1,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3puro-mGFP-TOP3B

    Plasmid

    #225470

    Purpose

    Express mEGFP-fusion protein of TOP3B

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    TOP3B (TOP3B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4.TO-ORF57-2xCSTREP

    Plasmid

    #136217

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF57
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-TO-PV-NES-DsRed

    Plasmid

    #16339

    Depositor

    Anton Bennett

    Article

    Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

    Insert

    PV-NES-DsRed (Pvalb Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only