We narrowed to 48,617 results for: Spr

1. pKDsgRNA-pheT

   Plasmid

   #154920

   Purpose

   aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting pheT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDsgRNA-mqo

   Plasmid

   #154921

   Purpose

   aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting mqo
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKDsgRNA-hisF

   Plasmid

   #154922

   Purpose

   aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting hisF
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKDsgRNA-ampG

   Plasmid

   #154923

   Purpose

   aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting ampG
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKDsgRNA-pheT

   Plasmid

   #154920

   Purpose

   aTc inducible gRNA targeting pheT along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting pheT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKDsgRNA-mqo

   Plasmid

   #154921

   Purpose

   aTc inducible gRNA targeting mqo along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting mqo
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKDsgRNA-hisF

   Plasmid

   #154922

   Purpose

   aTc inducible gRNA targeting hisF along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting hisF
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDsgRNA-ampG

   Plasmid

   #154923

   Purpose

   aTc inducible gRNA targeting ampG along with arabinose-inducible lambda red enzymes

   Depositor

   Michael Lynch

   Article

   Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

   Insert

   gRNA targeting ampG
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDCC6

   Plasmid

   #59985

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Inserts

   Cas9
   U6-2>sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   U6-96Ab and hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    U6-96Ab and hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWJ66

    Plasmid

    #46570

    Purpose

    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9-w
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWJ66

    Plasmid

    #46570

    Purpose

    Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9-w
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYZ1389

    Plasmid

    #215348

    Purpose

    Nla CRISPRi Delivery Vector

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    Nla CRISPRi Genes (Cas5, Cas8c, Cas7)
    

    Use

    CRISPR; Nla i delivery vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ1389

    Plasmid

    #215348

    Purpose

    Nla CRISPRi Delivery Vector

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    Nla CRISPRi Genes (Cas5, Cas8c, Cas7)
    

    Use

    CRISPR; Nla i delivery vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge