We narrowed to 47,149 results for: Spr

1. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-UMI

   Plasmid

   #222694

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PGK without BsmBI cutsite

   Promoter

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQCi-mB2m-sgRNA

   Plasmid

   #154090

   Purpose

   pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting murine B2M locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAH243

   Plasmid

   #121147

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   humanized Streptococcus pyogenes Cas9
   gRNA expression module
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCi-hTRAC-sgRNA

   Plasmid

   #154093

   Purpose

   pQCi backbone with sgRNA targeting human TCR-alpha common chain

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   sgRNA targeting human TRAC locus
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCT

   Plasmid

   #60620

   Purpose

   Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459V2.0-SpCas9-HF4

   Plasmid

   #108295

   Purpose

   pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQAT22

   Plasmid

   #223394

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT19

   Plasmid

   #223391

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT21

    Plasmid

    #223393

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQAT23

    Plasmid

    #223395

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRetro-UMI

    Plasmid

    #222686

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDG461

    Plasmid

    #100902

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEN017-SpCas9-hiNLS-t-M4

    Plasmid

    #234455

    Purpose

    Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY036_ATP1A1_G5_Array

    Plasmid

    #86620

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY036_ATP1A1_G3_Array

    Plasmid

    #86619

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge