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HL3953

Bacterial Strain

#37985

Depositor

Han Lim

Article

Liang et al Genetics. 2013 Feb;193(2):453-65. doi: 1

Bacterial Resistance

Kanamycin

Available Since

July 5, 2012

Availability

Academic Institutions and Nonprofits only
C. elegans miniMos transgenesis kit

Plasmid Kit

#1000000031

Purpose

29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.

Depositor

Erik Jorgensen

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only
Human Whole Genome sgRNA iBAR Library

Pooled Library

#140633

Purpose

Enables genome-wide CRISPR screening at high MOI through use of barcoded gRNA plasmids.

Depositor

Wensheng Wei

Article

Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

Use

CRISPR and Lentiviral

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197349

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Plasmid

#159810

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter cel-mir-43-44 context

Plasmid

#46654

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

cel-mir-43-44 context

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-145

Plasmid

#22291

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Synaptojanin 1-170

Plasmid

#22292

Depositor

Pietro De Camilli

Article

Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

K334R compared to Genbank ID NM_003895

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-170

Plasmid

#22294

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_170 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pmCh-N1 BLTP3A 1-1327

Plasmid

#241253

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-1327 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-1327 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A 1-1364

Plasmid

#241254

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-1364 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-1364 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A 1-336

Plasmid

#241250

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-336 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-336 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3 chimera-1

Plasmid

#241251

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) chimera-1 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) chimera-1 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest53-SHP-1 WT

Plasmid

#21791

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pQCXIP X2 DEST (w270-1)

Plasmid

#17474

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pQCXIN X2 DEST (w310-1)

Plasmid

#17399

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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