We narrowed to 13,611 results for: Mpl;

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pAAV-hSyn-mCherry.3xFLAG-WPRE

Plasmid

#127861

Purpose

pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

mCherry

Use

AAV

Tags

3xFLAG

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p240 pPr-CREM/Ins

Plasmid

#8405

Purpose

Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

probasin driven CREM with insulators

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin promoter

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-PPHLN1 ts1

Plasmid

#115877

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-MGD

Plasmid

#33033

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Mutation

MGD = amino acid substitutions M383I, G384S and D…

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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645 pCS2-etv2

Plasmid

#49005

Purpose

Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Invitro transcription, riboprobe template

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn4

Plasmid

#164960

Purpose

Zebrafish kcnn4 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn4 (kcnn4 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot10_K

Plasmid

#146775

Purpose

Mammalian Expression of HsNot10

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsNot10 (CNOT10 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Plasmid

#191394

Purpose

R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Mutation

R44Q

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-TO-HA-SH3BP1 (RNAi-res)

Plasmid

#136645

Purpose

Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pTYF-1xSYN-EGFP

Plasmid

#19973

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

SYN-EGFP-WPRE (SYN1 jellyfish, Human)

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1-del1174-1649_AH

Plasmid

#148904

Purpose

Mammalian Expression of HsXRN1-del1174-1649

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2019 Sep 26;47(17):92

Insert

HsXRN1-del1174-1649 (XRN1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-AthHEN1

Plasmid

#104980

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-DIO {ChETA}on-WPRE

Plasmid

#111386

Purpose

AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Insert

ChETA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

humanized ChR2, E123T/H134R

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76A

Plasmid

#115843

Purpose

Encodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMAC251-Vpx-Q76A

Tags

3x-FLAG

Expression

Mammalian

Mutation

Q76A

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-PAX9

Plasmid

#75079

Purpose

retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPC1/GgVcl 1-258 A50I mutation

Plasmid

#46271

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 (aka VD1) A50I mutation (VCL Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

1-258 (VD1 domain) A50I mutation

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-TEV-YFP-Flag

Plasmid

#41004

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

TEV-YFP-Flag

Expression

Mammalian

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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1099-N-cadherin-AAA-YFP

Plasmid

#32237

Depositor

Kathleen Green

Article

Chen et al J Cell Biol. 2003 Nov 10;163(3):547-57.

Insert

N-cadherin (Cdh2 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Glu–Glu–Asp (aa 780–782) ---> Ala–Ala–Ala

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pQsupR-Mi2b

Plasmid

#15379

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)

Use

RNAi and Retroviral

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pRVdG-4RFP

Plasmid

#52489

Purpose

Expresses TagRFP-T

Depositor

Sebastian Seung

Article

Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

Insert

TagRFP-T

Use

Rabies virus

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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p6903 PHAGE-B CMV-N-EGFP Myc

Plasmid

#37608

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

Myc (MYC Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Plasmid

#159866

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-pShuttle

Plasmid

#122122

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Tags

Omni

Expression

Mammalian

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

Plasmid

#136646

Purpose

Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1R312A (RNAi resistant) (SH3BP1 Human)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Myc-hAtg14

Plasmid

#24293

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Tags

3xMyc

Expression

Mammalian

Available Since

Sept. 15, 2010

Availability

Academic Institutions and Nonprofits only
MEIS1B-MSCV

Plasmid

#21022

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1999 Apr . 19(4):3051-61.

Insert

MEIS1B (Meis1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3 WT MYOC eGLuc2 FLAG

Plasmid

#192820

Purpose

Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

Depositor

John Hulleman

Article

Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

Insert

myocilin (MYOC Human)

Tags

FLAG and enhanced Gaussia luciferase (M43I/M110I)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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