We narrowed to 6,334 results for: nls
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Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM767
Plasmid#133604PurposePart Plasmid for dCas9 NLS, Part 3(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM768
Plasmid#133605PurposePart Plasmid for dCas9 NLS, Part 3b(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM769
Plasmid#133606PurposePart Plasmid for dCas9 NLS, Part 3a(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Intersectin SH3 domain A
Plasmid#47396DepositorInsertIntersectin SH3 domain A
TagsGSTExpressionBacterialMutationSH3 domain APromotertacAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH127
Plasmid#162483PurposeSEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH130
Plasmid#162484PurposeSEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsmyo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB–DualPE
Plasmid#235161PurposeFor plant prime editing including large DNA fragment editing in wheat plants or other monocotyledonsDepositorInsertsCsy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
CmYLCV-epegRNA-CaMV poly(A) signal
UseCRISPRMutationDetailed in manuscriptAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pErCas12a EZ Clone
Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#162482PurposeSEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.DepositorInsertvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
UseCRISPRTagsvha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTRExpressionWormAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-SpCas9
Plasmid#130968Purpose3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-SpCas9
UseCRISPRTags3x HA, EGFP, and NLSExpressionMammalianPromoterCbhAvailable SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
TagsMBP, hexa His, and two SV40 NLSExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCasRx-RBM25
Plasmid#221001PurposeTransient expression of dCasRx-RBM25. EF1a promoter.DepositorInsertdCasRx-RBM25
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterEF1aAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
enOgeuIscB with ωRNA-v2
Plasmid#220939PurposeCMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloingDepositorInsertsCMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
UseCRISPRExpressionMammalianAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-MYC
Plasmid#101765PurposeGateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion proteinDepositorTypeEmpty backboneTagsMycExpressionMammalianAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneTagsHAExpressionMammalianAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pprm1-luciferase; his-3
Plasmid#137728PurposeCommunication-activated luciferase in Neurospora crassaDepositorInsertsLuciferase
Pprm-1 Promoter
UseLuciferase; Neurospora crassa expressionMutationCodon-optimized for N. crassaAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4455 FLAG-hPLIC-2
Plasmid#8661DepositorAvailable SinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only