We narrowed to 31,194 results for: uros

1. pLVX-Flag-Polß(WT)-IRES-puro

   Plasmid

   #128653

   Purpose

   mammalian expression of Flag-Polß(WT) protein with puromycin selection

   Depositor

   Robert Sobol

   Article

   Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

   Insert

   PolB (POLB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-PGK-RAP1A-E63-puro

   Plasmid

   #156167

   Purpose

   Lentiviral vector for expression of RAP1A-E63, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Glutamine 63 to Glutamic acid

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC118-mCtnnb1-FLIP-FlpE-Puro

   Plasmid

   #84541

   Purpose

   CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

   Depositor

   Bon-Kyoung Koo

   Article

   Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

   Insert

   mouse beta-catenin FLIP-FlpE - Puro targeting vector
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pLC-Flag-CSNK1A1 G40N-Puro

   Plasmid

   #123320

   Purpose

   Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Glycine 40 to Asparagine

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiPGK Puro DEST JNKKTR EE Clover

   Plasmid

   #90239

   Purpose

   Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   JNK Kinase Translocation Reporter (EE mutant)
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEFS-ccdB-12X MS2_mPGK-Puro

   Plasmid

   #177809

   Purpose

   Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

   Type

   Empty backbone

   Use

   Tags

   12X MS2 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTopo pLox hPGK-Puro pA pLox

   Plasmid

   #171048

   Purpose

   Empty donor template for CRISPR/Cas9 targeting of TERT RE's

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

   Plasmid

   #68345

   Purpose

   A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   puromycin
   hCas9
   

   Use

   CRISPR; Flp/frt

   Tags

   linked to red flouresent protein gene mKateII and…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

   Plasmid

   #115519

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   ccdB-sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX puro TRIM21 C54Y - GFP

    Plasmid

    #116942

    Purpose

    Expression of TRIM21 C54Y -GFP fusion

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 C54Y-GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C54Y

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE puro Brca1 S1423A S1524A HA

    Plasmid

    #41968

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1423A, S1524A

    Promoter

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pUC57-PB-PGK-tetR-KRAB-2A-Puro

    Plasmid

    #129536

    Purpose

    PiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    PB-PGK-tetR-KRAB-2A-Puro
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    hPGK promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mis12-targted Aurora B FRET sensor

    Plasmid

    #45231

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    Mis12 targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-PtenK13E/K289E-Puro

    Plasmid

    #135681

    Purpose

    Constitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K13E/K289E

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe puro pI3K p110 CAAX

    Plasmid

    #13339

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    pI3K p110 CAAX (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pBabe-TetCMV-puro-mVenus-LOV

    Plasmid

    #22033

    Purpose

    Mammalian expression of mVenus tagged LOV2 domain

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-LOV
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Insert length based on Venus (720bp) + LOV (450bp)

    Promoter

    Available since

    Sept. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-TRE3G-BE3-PGK-Puro

    Plasmid

    #110845

    Purpose

    Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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