Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 3,019 results for: GRI;

Showing: 601 - 650 of 3019 results

  7. TOPO mMecp2-NLucTom

    Plasmid
    #159282
    Purpose
    To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.
    Depositor
    Joost Gribnau
    Article
    Mira-Bontenbal et al Stem Cell Reports. 2022 Jan 29. pii: S22
    Insert
    Mecp2 (Mecp2 Mouse)
    Use
    Tags
    Nluciferase-P2A-TdTomato
    Expression
    Mammalian
    Mutation
    Nluciferase-TdTomato fusion
    Promoter
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    159282-plasmid-map-sequence-id-313970

    Enlarge

  18. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Promoter
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

    Enlarge

  19. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Promoter
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

    Enlarge

  20. pcDNA3.1-GSDMD_29-484

    Plasmid
    #218231
    Purpose
    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-484
    Promoter
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218231-plasmid-map-sequence-id-436177

    Enlarge

  21. pcDNA3.1-GSDMD_29-270

    Plasmid
    #218232
    Purpose
    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Use
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 29-270
    Promoter
    Available since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218232-plasmid-map-sequence-id-430566

    Enlarge

  22. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

    Enlarge

  23. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

    Enlarge

  24. pAAV-hSyn-GRAB_OT1.0 (AAV1)

    Viral Prep
    #185386-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep
    #121675-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  26. pAAV-EF1a-fDIO-Cre (AAV1)

    Viral Prep
    #121675-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-EF1a-fDIO-Cre (AAV8)

    Viral Prep
    #121675-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-EF1a-fDIO-Cre (AAV5)

    Viral Prep
    #121675-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV-hSyn-GRAB_OT1.0 (AAV9)

    Viral Prep
    #185386-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  30. pAAV-hSyn-GRAB_OTmut (AAV1)

    Viral Prep
    #185388-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  31. pAAV-hSyn-GRAB_OTmut (AAV9)

    Viral Prep
    #185388-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Promoter
    Syn
    Tags
    None
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  32. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep
    #121675-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Alexander Nectow, Esteban Engel
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    May 31, 2019
    Availability
    Academic Institutions and Nonprofits only

  33. pAAV-hsyn-flex-Voltron-ST (AAV1)

    Viral Prep
    #119036-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Eric Schreiter
    Article
    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641
    Promoter
    Syn
    Tags
    None
    Available since
    April 10, 2019
    Availability
    Academic Institutions and Nonprofits only

  34. pAAV-CAG-SomaGCaMP6f2 (AAV8)

    Viral Prep
    #158757-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Promoter
    CAG promoter
    Tags
    None
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV-CAG-SomaGCaMP6f2 (AAV9)

    Viral Prep
    #158757-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Promoter
    CAG promoter
    Tags
    None
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  36. pGP-AAV-syn-FLEX-Volton2-ST-WPRE (AAV1)

    Viral Prep
    #172907-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Volton2-ST-WPRE (#172907). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Volton2-ST-WPRE plasmid DNA. Cre-dependent, Syn-driven expression of the voltage sensor Voltron2-ST containing a soma localization targeting sequence. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Promoter
    Syn
    Tags
    None
    Available since
    July 21, 2023
    Availability
    Academic Institutions and Nonprofits only

  37. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (AAV1)

    Viral Prep
    #172908-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE (#172908). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE plasmid DNA. Syn-driven, Cre-dependent Ace2N-4AA-mNeon-ST A122D expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-
    Promoter
    Syn
    Tags
    mNeon (Cre-dependent)
    Available since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  41. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Feng Zhang
    Insert
    huCPbCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92298-plasmid-map-sequence-id-181648

    Enlarge

  43. pRDB_345

    Plasmid
    #231113
    Purpose
    ABE activity-based selection cloning vector
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231113-plasmid-map-sequence-id-458424

    Enlarge

  44. pRDB_347

    Plasmid
    #231114
    Purpose
    CBE activity-based selection cloning vector
    Depositor
    David Root, John Doench
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    231114-plasmid-map-sequence-id-458384

    Enlarge

Showing: 601 - 650 of 3019 results