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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,231 results for: Aspa

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Showing: 241 - 260 of 1231 results

  3. N-terminal 3X Flag Human CASP9 C287A Mutant

    Plasmid
    #198380
    Purpose
    Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells
    Depositor
    Hannah Rabinowich
    Article
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Insert
    N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Cysteine 287 to Alanine to eliminate prot…
    Promoter
    CMV
    Available Since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198380-plasmid-map-sequence-id-390258

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  5. AAV-GFP/Cre

    Plasmid
    #49056
    Purpose
    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.
    Depositor
    Fred Gage
    Article
    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99
    Insert
    Cre
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    EGFP, Myc, and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49056-plasmid-map-sequence-id-182646

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  6. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Available Since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

    Plasmid
    #131256
    Purpose
    Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Contains point mutations that convert USP7 aspart…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131256-plasmid-map-sequence-id-267711

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  8. pcDNA3.1-GSDMD_1-275

    Plasmid
    #218230
    Purpose
    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-275
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218230-plasmid-map-sequence-id-430565

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  10. pAIP4_1ZHV

    Plasmid
    #234197
    Purpose
    Heterologous protein expression of aspartate kinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ZHV
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234197-plasmid-map-sequence-id-464162

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  11. pAIP4_5IHW

    Plasmid
    #234148
    Purpose
    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5IHW
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234148-plasmid-map-sequence-id-464114

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  15. pJK292

    Plasmid
    #73371
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changed histidine-59 to asparagine and histidine-…
    Promoter
    T7
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73371-plasmid-map-sequence-id-135107

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  16. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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Showing: 241 - 260 of 1231 results