We narrowed to 5,264 results for: pcdna3.1

1. pcDNA3.1_ADAR2DD_E488Q_Ago2

   Plasmid

   #205599

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   hAgo2 (AGO2 Human)
   

   Use

   Tags

   ADAR2DD_E488Q (hyperactive mutant)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1.D2-NP

   Plasmid

   #134366

   Purpose

   Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   D2-NP (DRD2 Human)
   

   Use

   Tags

   Flag, natural peptide of nanoluciferase, and sign…

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_hHNF4alpha

   Plasmid

   #206878

   Purpose

   The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

   Depositor

   Catharine Ross, Reza Zolfaghari

   Article

   Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

   Insert

   Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_RFP

   Plasmid

   #206177

   Purpose

   mammalian expression of RFP

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1alphaFEM1CalphaFLAG

   Plasmid

   #236462

   Purpose

   transient overexpression of FEM1C in mammalian cells

   Depositor

   Michele Pagano

   Article

   Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

   Insert

   FEM1C (FEM1C Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1hSIRT5_HA

   Plasmid

   #24483

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Sirtuin 5 (SIRT5 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_rSERT_WT

   Plasmid

   #190743

   Purpose

   For expression of wild-type rat SERT in mammalian cells

   Depositor

   Gary Rudnick

   Article

   Singh et al bioRxiv 2022.06.13.495991

   Insert

   SLC6A4 Serotonin Transporter (Slc6a4 Rat)
   

   Use

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   Shinwu Jeong, M. Elizabeth Fini

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Johannes Vierock, Peter Hegemann

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Use

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_PsChR2_EYFP

    Plasmid

    #69057

    Purpose

    High-efficiency blue-shifted cation channelrhodopsin

    Depositor

    John Spudich

    Article

    Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

    Insert

    PsChR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_ionRD

    Plasmid

    #172931

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Use

    Tags

    haemagglutinin

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1hSIRT3_H248Y_HA

    Plasmid

    #24492

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H248Y

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1_ionRE

    Plasmid

    #172932

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1hSIRT4_HA

    Plasmid

    #24482

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 4 (SIRT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_27F3_hFc

    Plasmid

    #199619

    Purpose

    Encodes DARPin-hFc 27F3 for mammalian expression and column purification

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    DARPin-hFc 27F3
    

    Use

    Tags

    hFc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_hE2F7mg

    Plasmid

    #73073

    Purpose

    human E2F7 minigene (exons 11,12,13/introns cassette)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    E2F7 (E2F7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    partial gene, exons 11,12,13 spaced by introns

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1.H1-NP

    Plasmid

    #134367

    Purpose

    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    H1-NP (HRH1 Human)
    

    Use

    Tags

    Flag and natural peptide of nanoluciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge