We narrowed to 5,336 results for: pcdna3.1

1. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_ADAR2DD_E488Q_Ago2

   Plasmid

   #205599

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   hAgo2 (AGO2 Human)
   

   Tags

   ADAR2DD_E488Q (hyperactive mutant)

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Johannes Vierock, Peter Hegemann

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1hSIRT3_H248Y_HA

   Plasmid

   #24492

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H248Y

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_RFP

   Plasmid

   #206177

   Purpose

   mammalian expression of RFP

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   RFP
   

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_hHNF4alpha

   Plasmid

   #206878

   Purpose

   The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

   Depositor

   Reza Zolfaghari, Catharine Ross

   Article

   Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

   Insert

   Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   Shinwu Jeong, M. Elizabeth Fini

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HcKCR1_mCherry_pcDNA3.1

   Plasmid

   #177336

   Purpose

   Expresses KCR1 in mammalian cells

   Depositor

   John Spudich

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR1
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   mammalian codon-adapted

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. GtCCR2_EYFP_pcDNA3.1

   Plasmid

   #159121

   Purpose

   This plasmid encodes GtCCR2 rhodopsin domain

   Depositor

   John Spudich

   Article

   Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

   Insert

   GtCCR2
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_hCAR.Variant3

    Plasmid

    #209133

    Purpose

    The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Insert

    constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1.D2-NP

    Plasmid

    #134366

    Purpose

    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    D2-NP (DRD2 Human)
    

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_SybBC10

    Plasmid

    #240997

    Purpose

    Expression of sybody SLC66A1 in mammalian cells

    Depositor

    Simon Newstead

    Insert

    Syb-BC10-Myc-His6
    

    Tags

    Myc and His6

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_PsChR2_EYFP

    Plasmid

    #69057

    Purpose

    High-efficiency blue-shifted cation channelrhodopsin

    Depositor

    John Spudich

    Article

    Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

    Insert

    PsChR2
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1alphaFEM1CalphaFLAG

    Plasmid

    #236462

    Purpose

    transient overexpression of FEM1C in mammalian cells

    Depositor

    Michele Pagano

    Article

    Dankert et al Cell Cycle. 2017 Mar 19;16(6):556-564. d

    Insert

    FEM1C (FEM1C Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Tags

    haemagglutinin

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_ionRD

    Plasmid

    #172931

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_rSERT_WT

    Plasmid

    #190743

    Purpose

    For expression of wild-type rat SERT in mammalian cells

    Depositor

    Gary Rudnick

    Article

    Singh et al bioRxiv 2022.06.13.495991

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1hSIRT4_HA

    Plasmid

    #24482

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 4 (SIRT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_ionRE

    Plasmid

    #172932

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRE
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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