Addgene

We narrowed to 6,474 results for: sas

Showing: 241 - 260 of 6474 results

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Plasmid

#182124

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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p102_gRNA_Sa_mClover

Plasmid

#213778

Purpose

Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA

Plasmid

#197044

Purpose

In situ cell-surface proteome extraction by extracellular labeling (iPEEL)

Depositor

Liqun Luo

Article

Shuster et al Neuron. 2022 Dec 7;110(23):3882-3896.e9.

Inserts

pCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA

Use

Tags

HA, myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJDS78-GG-SNG

Plasmid

#44716

Depositor

Nathan Lawson

Article

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Use

Tags

3X Flag and FOKI WT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pJDS74-GG-NNN

Plasmid

#44715

Depositor

Nathan Lawson

Article

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Use

Tags

3X Flag and FOKI WT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pJDS70-GG-SNI

Plasmid

#44713

Depositor

Nathan Lawson

Article

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Use

Tags

3X Flag and FOKI WT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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pJDS71-GG-SHD

Plasmid

#44714

Depositor

Nathan Lawson

Article

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Use

Tags

3X Flag and FOKI WT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Article

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Tags

Expression

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pH3U3-Zif268

Plasmid

#12610

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Insert

Zif268 binding site in pH3U3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR_221_middle-entry_NanoLuc

Plasmid

#128673

Purpose

NanoLuc CDS middle entry gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pUCKanR-Mu-BsaI

Plasmid

#79769

Purpose

Transposon-propagation plasmid for Mu-BsaI modified transposon

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Mu-BsaI

Use

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pATT-Dest

Plasmid

#79770

Purpose

Staging plasmid for use in DIP transposition

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Synthetic Biology; Staging plasmid for transposit…

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTKEI-Dest

Plasmid

#79784

Purpose

Expression plasmid - BsmBI entry sites

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Type

Empty backbone

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTKEI-tLOV

Plasmid

#100574

Purpose

Expression plasmid for the thermostable anaerobic fluorescent protein tLOV

Depositor

David Savage

Article

Higgins et al ACS Synth Biol. 2017 Jul 24. doi: 10.102

Insert

tLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTrc

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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9336-E21

Plasmid

#234304

Purpose

Gateway ORF Entry clone of human SMAD3 EPSM-SA with stop codon (for native or N-terminal fusions); Linker and C-terminal sites all dead

Depositor

Dominic Esposito

Insert

SMAD3 EPSM-SA (SMAD3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

T179V/S204A/S208A/S213A/S422A/S423A/S425A; Linker…

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMV762-Peredox-mCherry

Plasmid

#90217

Purpose

Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

Depositor

Ashwani Kumar

Article

Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

Insert

Codon Optimized Peredox-mCherry

Use

Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

groEL

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-N1

Plasmid

#54662

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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