We narrowed to 2,439 results for: sds

Showing: 241 - 260 of 2439 results

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hHSF1 K80R

Plasmid

#118350

Purpose

Plasmid expresses hHSF1 with K80 in DBD mutated to R.

Depositor

Lea Sistonen

Article

Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K80R

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Lyn11C32S-PIP4K2A

Plasmid

#202748

Purpose

weakly plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2_Renilla_luc-60A

Plasmid

#200397

Purpose

A plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Renilla luciferase

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1(118-1274)

Plasmid

#221207

Purpose

ScChd1-aa118-1274 chromatin remodeler

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-eDIO-EYFP

Plasmid

#104388

Purpose

PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

Depositor

Volker Busskamp

Article

Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

Insert

Enhanced Yellow Fluorescent Protein

Use

Cre/Lox; Piggybac

Expression

Mammalian

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pROSA26-FlpERT2

Plasmid

#149438

Purpose

ROSA26 mouse targeting vector with tamoxifen-inducible Flp recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Flp-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB S28N

Plasmid

#19722

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

S28N for dominant negative function.

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pMT3-FLAG-KLF3

Plasmid

#49102

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Himeda et al Mol Cell Biol. 2010 Jul;30(14):3430-43.

Insert

Krüppel-Like Factor 3(KLF3)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe puro RalB Q72L

Plasmid

#19721

Depositor

Channing Der

Article

Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

Insert

V-ral simian leukemia viral oncogene homolog B (RALB Human)

Use

Retroviral

Expression

Mammalian

Mutation

Q72L constitutively active, GTPase Deficient

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP-60A

Plasmid

#200394

Purpose

Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

RFP

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-14-3-3 Beta

Plasmid

#19957

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

14-3-3 Protein Beta (YWHAB Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pGEX2TK-Osh2-PH

Plasmid

#71370

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pGEX2TK-Num1-PH

Plasmid

#71369

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBI-GFP-Bub1-S492Y

Plasmid

#16625

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Insert

BUB1 S492Y (BUB1 Human)

Expression

Mammalian

Mutation

S492Y

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh1-PH

Plasmid

#71372

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa267-388

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pGEX2TK-Skm1-PH

Plasmid

#71371

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Skm1-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa1-132

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

Plasmid

#13006

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 deltaCC (SULF2 Human)

Expression

Mammalian

Mutation

changed Cysteine 88 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only