Addgene

We narrowed to 23,664 results for: spr

Showing: 241 - 260 of 23664 results

Results per page:

Sort results by:

pYPQ166-SpRY

Plasmid

#161520

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

Depositor

Yiping Qi

Article

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9

Use

CRISPR; Gateway compatible zsprycas9 entry clone

Tags

3X FLAG, NLS and NLS

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR v2 hBAK

Plasmid

#129579

Purpose

CRISPR deletion of human BAK

Depositor

Stephen Tait

Article

Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

Insert

gRNA vs human BAK (BAK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_full-site

Plasmid

#186277

Purpose

Reporter plasmid for full-site selection assay

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-TKT_sgRNA2

Plasmid

#201625

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-TKT_sgRNA1

Plasmid

#201624

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-S12

Plasmid

#84031

Purpose

To episomally express codon optimized Cas9 and chimeric guide RNA

Depositor

Sen Wu

Article

Li et al Protein Cell. 2016 Sep;7(9):689-91. doi:

Inserts

hSpCas9
eGFP

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Promoter

CMV and CMV (downstream of F2A self-cleaving pept…

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pYLCRISPR/Cas9pUbi-N

Plasmid

#106331

Purpose

Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

ZmUbi

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPRainbow-DONOR1

Plasmid

#75398

Purpose

CRISPRainbow-DONOR1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Cm(R)-CcdB

Use

CRISPR

Tags

no

Expression

Mutation

Promoter

Bacteria

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgACSL4

Plasmid

#186024

Purpose

knock out ACSL4 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCRISPRed pRSETB

Plasmid

#159454

Purpose

Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Use

Tags

10 x His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv1-sgAAVS1

Plasmid

#83906

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPR-sgPREX1

Plasmid

#86127

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

gRNA targeting PREX1 (PREX1 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
mSprouty1/3B14

Plasmid

#22091

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 1 (Spry1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2-KLF7-1

Plasmid

#169797

Purpose

Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

guide RNA for KLF7 neighbouring region

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgHK2_2

Plasmid

#163461

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 2 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-HK2_sgRNA1

Plasmid

#201596

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge