Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 6,562 results for: nls

Clear keywords
Showing: 2581 - 2600 of 6562 results

  1. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220613
    Purpose
    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220613-plasmid-map-sequence-id-441115

    Enlarge

  2. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220608
    Purpose
    Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220608-plasmid-map-sequence-id-441203

    Enlarge

  3. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

    Plasmid
    #220614
    Purpose
    Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
    Use
    AAV and Cre/Lox
    Tags
    3x HA; ArgiNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220614-plasmid-map-sequence-id-441114

    Enlarge

  4. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

    Plasmid
    #230043
    Purpose
    Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.
    Depositor
    Sumeet Pal Singh
    Publication
    Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:
    Insert
    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    fabp10a
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    230043-plasmid-map-sequence-id-461059

    Enlarge

  5. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Publication
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

    Enlarge

  10. NLS-LucWT-GFP

    Plasmid
    #127709
    Purpose
    The expressed ORF encodes nucleus location signal with GFP and WT Luciferase.
    Depositor
    Xiaolu Yang
    Publication
    Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luciferase
    Tags
    EGFP and FLAG-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    127709-plasmid-map-sequence-id-248776

    Enlarge

  11. NLS-LucDM-GFP

    Plasmid
    #127796
    Purpose
    The expressed ORF encodes nucleus location signal with GFP and destabilized mutant Luciferase.
    Depositor
    Xiaolu Yang
    Publication
    Guo et al J Vis Exp. 2016 Aug 28;(114). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    destabilized mutant (DM) Luciferase
    Tags
    EGFP and FLAG-NLS
    Expression
    Mammalian
    Mutation
    R188Q, R261Q in Firefly Luciferase insert
    Promoter
    CMV
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127796-plasmid-map-sequence-id-248775

    Enlarge

  13. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

    Enlarge

  14. mEGFP-PCNA-19-SV40NLS-4

    Plasmid
    #56469
    Purpose
    Localization: Nucleus, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  15. pEG302 5aa SunTag VP64 g4+g17 (FWA)

    Plasmid
    #119672
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    119672-plasmid-map-sequence-id-242023

    Enlarge

  16. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid
    #228558
    Purpose
    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus
    Depositor
    Neville Sanjana
    Publication
    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103
    Insert
    dhfCas13d (N2V8)
    Use
    Lentiviral
    Tags
    NLS-HA
    Mutation
    R239A/H244A/R858A/H863A
    Available since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    228558-plasmid-map-sequence-id-457180

    Enlarge

  18. pEG302 22aa SunTag VP64 gRNA17 (FWA)

    Plasmid
    #120250
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120250-plasmid-map-sequence-id-238538

    Enlarge

Showing: 2581 - 2600 of 6562 results