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AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

Viral Prep

#51084-AAV1

Purpose

Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

Depositor

Jonathan Ting

Promoter

Syn

Tags

nuclear dTomato (physically separate, not a fusion protein)

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
HB401: pMVP (L1-L4) CMV promoter

Plasmid

#121686

Purpose

pMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJTX-GL3

Plasmid

#11981

Purpose

Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pYES2-Ub-M-GFP

Plasmid

#11952

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-M-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pJSTX-GL3

Plasmid

#11982

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

Plasmid

#55219

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pEM3 = ncs-1p::nCre

Plasmid

#24034

Depositor

Cori Bargmann

Article

Macosko et al Nature. 2009 Apr 30. 458(7242):1171-5.

Insert

ncs-1 (ncs-1 Nematode)

Use

Cre/Lox

Tags

nCre

Expression

Worm

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCOCK CMVwt

Plasmid

#46559

Purpose

Mammalian expression plasmid with EGFP expressed from CMV wild-type promoter.

Depositor

Clifford Wang

Article

Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.

Insert

CMV wt promoter

Use

Synthetic Biology

Expression

Mammalian

Mutation

Our version has a G at base 724 (reference has C)

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

Plasmid

#110054

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pEMS1277

Plasmid

#29140

Purpose

Plasmid described in Yang et al., Genomics, 2009 (PMID: 18950699).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

CAG promoter

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c

Plasmid

#107599

Purpose

An entry vector with U6a and U6c promoter driving guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Inserts

U6a promoter
U6c promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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CAG::TdTomato

Plasmid

#140504

Purpose

Encodes TdTomato driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

CAG promoter

Use

Chicken expression

Expression

Mammalian

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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YCe1759 HC_Kan_CAGp_p3

Plasmid

#100672

Purpose

part designed to occupy position 3 of EMMA. Functional category: Constitutional promoter

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

CAG promoter

Use

Synthetic Biology

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3.1-Luc

Plasmid

#106457

Purpose

luciferase reporter, CMV promoter

Depositor

Georg Weber

Article

He et al Biochem Biophys Res Commun. 2004 Aug 13;

Insert

luciferase reporter, CMV promoter

Use

Luciferase

Expression

Mammalian

Mutation

WT promoter

Available Since

Nov. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

Plasmid

#24584

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Available Since

May 18, 2010

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-6

Plasmid

#14969

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/+334) (MYC Human)

Use

Luciferase

Expression

Mammalian

Mutation

-109/+334 (relative to the P2 transcription initi…

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only