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pRS416 Gal-RNQ1 153-405-YFP

Plasmid

#18689

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 153-405

Tags

YFP

Expression

Yeast

Mutation

Prion domain of Rnq1

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pILGFP9E3

Plasmid

#185842

Purpose

Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_12

Plasmid

#183061

Purpose

Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_10

Plasmid

#183059

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_9

Plasmid

#183058

Purpose

Biosensor for acetic acid: BM3R1-Haa1-mTurquoise2 under HAA1p and mCherry under BM3R1ENO1cp with flanks for genome integration in toHO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_11

Plasmid

#183060

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

HAA1 and Synthetic promoter

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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tdEos-Vinculin-14

Plasmid

#57691

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Vinculin (VCL Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNTI757 P(PGK1)-citrine P(HIS4)-mCherry

Plasmid

#164917

Purpose

Divergent promoter expression cassette, P(HIS4) expression with P(PGK1) normalizer

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(PGK1)-citrine and P(HIS4)-mCherry

Expression

Yeast

Promoter

PGK1 (yeast) and HIS4 (yeast)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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JBEI-6823

Plasmid

#84986

Purpose

For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-PMKco-Mkco

Expression

Bacterial

Promoter

LacUV5

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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JBEI-6833

Plasmid

#84983

Purpose

Expression of NudB (E. coli) and PMD (S. cerevisiae) are under control of Trc promoter for isopentenol production.

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

NudB-PMD

Expression

Bacterial

Promoter

trc promoter

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-pol30(K127/164R)-3'

Plasmid

#99549

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pTJK517

Plasmid

#121474

Purpose

CEN TRP1 CNA1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1

Use

Yeast cen vector

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM3193

Plasmid

#113390

Purpose

Alpha factor inducible Ty3 with his3AI

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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gRNA_tdtomato_reporter_1_ST1_2x_binding

Plasmid

#79368

Purpose

Assays activity of transcriptional activators fused to ST1 Cas9

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

tdtomato

Use

CRISPR

Expression

Mammalian

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHL 1454

Plasmid

#53019

Purpose

AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

fimB

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pGEMT-NRTN

Plasmid

#119896

Purpose

Mouse NRTN In situ hybridization probe

Depositor

Wenqin Luo

Article

Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

Insert

neurturin probe (Nrtn Mouse)

Use

In situ hybridization probe

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_653-EYFP

Plasmid

#111079

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_653

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Expression

Bacterial

Mutation

AT-swapped mutant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5-Flag-zEPLIN

Plasmid

#40957

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN (lima1 Zebrafish)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
mEos2-Talin-ABM-10

Plasmid

#57421

Purpose

Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin AMB

Tags

mEos2

Expression

Mammalian

Mutation

Actin Binding Module; I/LWEQ = 197 amino acids fr…

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHL 1916

Plasmid

#53023

Purpose

AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Insert

fimB

Use

Synthetic Biology

Tags

Asp terminator and RBS(st7)

Expression

Bacterial

Promoter

pLtetO-1

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEP0001_pIVTRup(BsmBI)_EGFP

Plasmid

#242543

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EGFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-NatMX6

Plasmid

#240972

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; NatMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO286

Plasmid

#235730

Purpose

Protein expression of yeast VPS15 (1-981)-ZZ in yeast

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

aa 1-981

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-BleMX6

Plasmid

#240974

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; BleMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

BleMX6

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-His3MX6

Plasmid

#240971

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HIS3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI_Fsx1-V5_GFPnes

Plasmid

#240503

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

Fsx1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn1-DIO-Flpo

Plasmid

#237222

Purpose

For Cre-dependent expression of Flp recombinase

Depositor

Thomas Hnasko

Article

Oriol et al bioRxiv [Preprint]. 2025 Jan 17:2024.06.

Insert

Flpo recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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