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We narrowed to 3,215 results for: N-EGFP

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Showing: 2601 - 2620 of 3215 results

  8. pGG422_UV5

    Plasmid
    #165607
    Purpose
    Vector for expression of the SpCas9 KG variant with sgRNA in E. coli: KG(SpCas9, D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 KG(D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1332K and R1333G mutations in SpCas9
    Promoter
    lacUV5 driving Cas9 KG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165607-plasmid-map-sequence-id-330962

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  10. pGG198

    Plasmid
    #165605
    Purpose
    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFP
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    hEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Secondary protospacer ('CGGCG' PAM) ins…
    Promoter
    lac
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    165605-plasmid-map-sequence-id-338038

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  17. pET15b-eGO-Matryoshka-LE-LP

    Plasmid
    #100017
    Purpose
    Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    cpEGFP-LSSmOrange
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Leu and Glu N-term linker and Leu and Pro C-term …
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100017-plasmid-map-sequence-id-194293

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  18. pGG426_UV5

    Plasmid
    #165608
    Purpose
    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1135V, S1136R, D1332K and R1333G mutations in Sp…
    Promoter
    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165608-plasmid-map-sequence-id-330965

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  19. pDL023_Tub-rb_IgG_HC

    Plasmid
    #193620
    Purpose
    α-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-Tubulin scFv heavy variable + rabbit constant chain
    Tags
    signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193620-plasmid-map-sequence-id-380455

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  20. pDL024_Tub-rb_IgG_LC

    Plasmid
    #193621
    Purpose
    α-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.
    Depositor
    Jennifer DeLuca
    Article
    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:
    Insert
    Anti-Tubulin scFv light variable + rabbit constant chain
    Tags
    signal peptide
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193621-plasmid-map-sequence-id-380456

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Showing: 2601 - 2620 of 3215 results