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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-SNAP-CD86iso2

Plasmid

#223574

Purpose

Lentiviral expression of human CD86 isoform 2 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PD-1 I126A

Plasmid

#223573

Purpose

Lentiviral expression of human PD-1 (I126A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

I126A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pI pm

Plasmid

#218098

Purpose

Phagemid that expresses LacI, and pI under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pI

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C G83R

Plasmid

#184747

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, G83R

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C Y78F

Plasmid

#184746

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, Y78F

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C A81T

Plasmid

#184745

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, A81T

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C K80E

Plasmid

#184744

Purpose

bacterial expression of huamn TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, K80E

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C I84S

Plasmid

#184748

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, I84S

Promoter

T7

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-10-mCherry-R11b

Plasmid

#179891

Purpose

Donor with homologous arms for Rab11b to knock in mCherry

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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HASP_HUMAN_D0

Plasmid

#79730

Purpose

This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

HASP

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2D_HUMAN_D0

Plasmid

#79734

Purpose

This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2D

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
AAPK2_HUMAN_D0

Plasmid

#79738

Purpose

This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AAPK2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC1G_HUMAN_D0

Plasmid

#79745

Purpose

This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC1G

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2A_HUMAN_D0

Plasmid

#79746

Purpose

This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2A

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1G2_HUMAN_D0

Plasmid

#79716

Purpose

This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK03_HUMAN_D0

Plasmid

#79688

Purpose

This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK03

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only