We narrowed to 12,012 results for: pcdna

1. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MAP3K5 (R256C)

   Plasmid

   #47105

   Purpose

   Expresses mutant (R256C) human MAP3K5 in mammalian cells

   Depositor

   Yardena Samuels

   Insert

   MAP3K5 (MAP3K5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed arginine 256 to cysteine

   Promoter

   CMV

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-ccdB-Firefly

   Plasmid

   #87066

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AktAR-Kras

   Plasmid

   #123357

   Purpose

   FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR-Kras
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-3xFLAG-RSL1D1(WT)

   Plasmid

   #122301

   Purpose

   expresses RSL1D1 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RSL1D1 (RSL1D1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.T7 delta-NFAT3

   Plasmid

   #28224

   Depositor

   Sebastien Jauliac

   Article

   Fougere et al Oncogene. 2010 Apr 15. 29(15):2292-301.

   Insert

   Delta NFAT3 (NFATC4 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   hNFAT3 WT deleted amino acids 1-342

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-AktAR2-NLS

   Plasmid

   #175012

   Purpose

   Nuclear-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   AktAR-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-p37-Strep-HA

   Plasmid

   #113485

   Purpose

   Expression of human p37 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   p37 (UBXN2B Human)
   

   Use

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT-TO-Nsp10

   Plasmid

   #157706

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp10 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA5/FRT/TO V5 DNAJA4

    Plasmid

    #19521

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily A, member 4 (DNAJA4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcdna6.2- myc ULK1 wt

    Plasmid

    #27629

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-FLAG-CyclinF-ΔF

    Plasmid

    #32973

    Depositor

    Michele Pagano

    Article

    D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

    Insert

    CYCLIN F (CCNF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F-box mutations: L35A and P36A

    Promoter

    CMV

    Available Since

    Feb. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCDNA6-LRRC26-V5-His

    Plasmid

    #127817

    Purpose

    Expression of human LRRC26 in mammalian cells

    Depositor

    Jiusheng Yan

    Article

    Li et al J Gen Physiol. 2015 Jun;145(6):543-54. d

    Insert

    LRRC26 (LRRC26 Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO HSPA2

    Plasmid

    #19458

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA2 (HSPA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag Myb

    Plasmid

    #66980

    Purpose

    Expression plasmid for mouse Myb; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Myb (Myb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5-FRT-TO-Nsp9-HF

    Plasmid

    #157705

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp9-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3-Ha-Brca1 S1423A

    Plasmid

    #32533

    Depositor

    Michael Kastan

    Article

    Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S1423A

    Promoter

    CMV/rabbit B-globin

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid

    #92306

    Purpose

    Expression of humanized BsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT-TO-FH-Nprot

    Plasmid

    #157729

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nprot (N SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA5-FRT-TO-FH-Nsp9

    Plasmid

    #157704

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp9 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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