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pET32a-HD25Q

Plasmid

#11508

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 25 glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 25 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-150

Plasmid

#26310

Depositor

David Bartel

Publication

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-150 (MIR150 Human)

Expression

Mammalian

Available since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Publication

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Available since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
PT2/C-fluc

Plasmid

#20203

Depositor

John Ohlfest

Publication

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C-Fluc

Use

Transposon

Available since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 S6K2 E388 D3E

Plasmid

#17731

Depositor

John Blenis

Publication

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

S6K2 (RPS6KB2 Human)

Tags

HA

Expression

Mammalian

Mutation

T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

Promoter

CMV

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG ARMS

Plasmid

#24086

Depositor

Moses Chao

Publication

Kong et al J Neurosci. 2001 Jan 1. 21(1):176-85.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ARMS (Kidins220 Rat)

Tags

FLAG

Expression

Mammalian

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET24c(+)-EFTu

Plasmid

#31172

Depositor

Linda Spremulli

Publication

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Tu (tufB E. coli)

Tags

His

Expression

Bacterial

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pET51b(+)_ALFA-tag-SNAP

Plasmid

#136628

Purpose

Plasmid encoding the bacterial expression of ALFA-tag asa SNAP-tag fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowR

Plasmid

#132668

Purpose

Expresses ShadowR in mammalian cells

Depositor

Hideji Murakoshi

Publication

Murakoshi et al Sci Rep. 2019 Aug 19;9(1):12072. doi: 10

Insert

ShadowR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTriExEKAR2G2

Plasmid

#40175

Depositor

Olivier Pertz

Publication

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-IP-EGFP-ULK1(K46N)

Plasmid

#38197

Depositor

Noboru Mizushima

Publication

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 46 to Asparagine

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEBG-hUBP43

Plasmid

#12455

Depositor

Dong-Er Zhang

Publication

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (USP18 Human)

Tags

GST

Expression

Mammalian

Mutation

pEBG vector is from Bruce Mayer, may be different…

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pRN3P_EGFP_m6A_Tracer

Plasmid

#139403

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

m6A-Tracer

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LeGO-Cer

Plasmid

#27351

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, Cerulean

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

Plasmid

#194457

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Publication

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-CD19 CAR

Use

Lentiviral

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD-MSP1D1

Plasmid

#161607

Purpose

Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Publication

McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

Insert

D-MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

mutant of MSP1D1; all tryptophans were replaced b…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Publication

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Publication

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Publication

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Expression

Mammalian

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article