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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9m4

Plasmid

#47316

Purpose

Cas9 D10A+D839A+H840A+N863A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m4

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b

Plasmid

#187362

Purpose

Expresses rat myosin 1b isoform b labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.GFP.miR9T

Plasmid

#115973

Purpose

miR-9 regulated GFP expression

Depositor

Johan Jakobsson

Article

Akerblom et al Nat Commun. 2013;4:1770. doi: 10.1038/nc

Insert

GFPmiR9T

Use

Lentiviral

Tags

GFP

Promoter

PGK

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Wnk1

Plasmid

#38779

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Tags

his-tag to facilitate purification

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCWori-A13AMO-aaCPRct

Plasmid

#20117

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

Amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

H2B-LSSmKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-Rab5

Plasmid

#51612

Purpose

Recruiter to Rab5-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab5

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLSSmKate1-C1

Plasmid

#31868

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSS-mKate1

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

mVenus-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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pPAmKate-N1

Plasmid

#34587

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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Double UP Halotag to mScarlet

Plasmid

#125138

Purpose

Natively produces Halotag, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-Rab7

Plasmid

#51613

Purpose

Recruiter to Rab7-positive endosomes

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

FRB
Rab7

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA13-GG

Plasmid

#208002

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SPS-7942

Plasmid

#80459

Purpose

IPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942

Depositor

Daniel Ducat

Article

Abramson et al Plant Cell Physiol. 2016 Oct 13. pii: pc

Insert

Sucrose phosphate synthase

Expression

Bacterial

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Gephyrin (px330:2-guideCas9)

Plasmid

#169432

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRL-ubi63E

Plasmid

#74280

Purpose

Renilla control plasmid driven by the ubiquitin 63E promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Insert

Renilla luciferase

Expression

Insect

Promoter

ubiquitin 63E promoter

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only

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Double UP mScarlet to mNeon

Plasmid

#125139

Purpose

Natively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Type

Empty backbone

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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